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BG-11 (Blue-green) Medium Solution 50x
Product ID: B1411


Description: BG-11 Medium is a freshwater growth medium for cyanobacteria (e.g., Synechocytis spp. PCC 6803). This is a 50x concentrated solution.

Miscibility Water
Physical Form Liquid
Powder Form B1411
Concentration 50x
Sterility Sterile Filtered
Storage Temp. 2-6 °C
UPC / SKU B1411
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
B210 Boric Acid
C266 Calcium Chloride, Anhydrous
C193 Cobalt Nitrate, Hexahydrate 2.45
C375 Cupric Sulfate, Pentahydrate 3.95
F374 Ferric Ammonium Citrate 300
M200 Magnesium Sulfate, Heptahydrate 3750
M455 Manganese Chloride, Tetrahydrate 90.5
P705 Potassium Phosphate, Dibasic 2000
S7550 Sodium Carbonate, Anhydrous 1000
M651 Sodium Molybdate (VI), Dihydrate 19.5
S802 Sodium Nitrate 75000
Z101 Zinc Sulfate, Heptahydrate 11.1
C277 Citric Acid, Anyhdrous 300
E2718 Disodium Magnesium EDTA 50

B1411 BG-11 Medium Solution, 50x

Synonyms: Blue-Green (BG) Medium
Form: Liquid
Appearance: Light Yellow to Yellow
Application: Cyanobacteria Media
Solubility: Miscible with Water
Typical Working Concentration: 20.00 mL/L
Storage Temp: 2-6ºC
Storage Temp of Stock Solution: 2-6ºC
Biological Assasy: Biologically tested with Synechocystis sp. PCC 6803.
Solution pH is approx. 4.2 +/- 0.5
Other Notes: It is suggested not to autoclave BG-11, 50x concentrate solution as precipitation may result.
Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222

Application Notes
BG-11 is generally a freshwater growth medium for culturing Cyanobacteria such as Synechocystis sp. PCC 6803.

HEPES - H326 (Akira et al. 1996) or TES (Xu et al. 1994) can be added to buffer the pH. After dissolving the powder medium, adjust to pH 8.0 with KOH or NaOH, and autoclave.

20 mM Hepes (H326) was used during biological testing of this product with Synechocystis sp. PCC 6803.

Akira, K., K. Lee, H. Fukuzawa, K. Ohyama, and T. Ogawa. (1996) cemA homologue essential to CO2 transport in the cynanobacterium Synechocystis PCC 6803. Proceedings of the National Academy of Sciences. 93:4006-4010.
Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1-61.
Stanier, R.Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazire. (1971) "Purification and Properties of Unicellular Blue-Green Algae (Order Chroococcales)." Bacteriological Reviews 35(2):171-205.
Xu, H., D. Vavilin, C. Funk, and W. Vermaas. "Multiple Deletions of Small-Cab-like Proteins.