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BG-11 (Blue-green) Medium
Product ID: B1511


Description: A freshwater standard growth medium for cyanobacteria (e.g., Synechocytis spp. PCC 6803).

Solubility Water
Physical Form Solid
Solutions Available B1411 (50x)
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 1.68
UPC / SKU B1511
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
B210 Boric Acid 2.86
C266 Calcium Chloride, Anhydrous 27.18
C193 Cobalt Nitrate, Hexahydrate 0.049
C375 Cupric Sulfate, Pentahydrate 0.079
F374 Ferric Ammonium Citrate 6
M200 Magnesium Sulfate, Heptahydrate 75
M455 Manganese Chloride, Tetrahydrate 1.81
P705 Potassium Phosphate, Dibasic 40
S7550 Sodium Carbonate, Anhydrous 20
M651 Sodium Molybdate (VI), Dihydrate 0.39
S802 Sodium Nitrate 1500
Z101 Zinc Sulfate, Heptahydrate 0.222
C277 Citric Acid, Anyhdrous 6.0
E2718 Disodium Magnesium EDTA 1.0

B1511 BG-11 Medium

Synonyms: Blue-Green (BG) Medium
Form: Powder
Appearance: Cream
Application: Cyanobacteria Growth Medium
Solubility: Soluble in Water
Typical Working Concentration: 1.68 g/L
Storage Temp: 2-6ºC
Storage Temp of Stock Solution: 2-6ºC
Biological Assay: Cell culture tested with Synechocystis sp. PCC 6803.
Formula (mg/L)
Boric Acid 2.86
Manganese Chloride·4H2O 1.81
Calcium Chloride Anhydrous 27.18
Na2·Mg·EDTA 1
Citric Acid, Anhydrous 6
Potassium Phosphate dibasic 40
Cobalt Nitrate·6H2O 0.049
Sodium Carbonate Anhydrous 20
Cupric Sulfate·5H2O 0.079
Sodium Molybdate·2H2O 0.39
Ferric Ammonium Citrate 6
Sodium Nitrate 1500
Magnesium Sulfate·7H2O 75
Zinc Sulfate*7H2O 0.222

Application Notes
BG-11 is generally a freshwater growth medium for culturing Cyanobacteria such as Synechocystis sp. PCC 6803.

HEPES - H326 (Akira et al. 1996) or TES (Xu et al. 1994) can be added to buffer the pH. After dissolving the powder medium, adjust to pH 8.0 with KOH or NaOH, and autoclave.

20 mM Hepes (H326) was used during biological testing of this product with Synechocystis sp. PCC 6803.

Akira, K., K. Lee, H. Fukuzawa, K. Ohyama, and T. Ogawa. (1996) cemA homologue essential to CO2 transport in the cynanobacterium Synechocystis PCC 6803. Proceedings of the National Academy of Sciences. 93:4006-4010.
Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1-61.
Stanier, R.Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazire. (1971) "Purification and Properties of Unicellular Blue-Green Algae (Order Chroococcales)." Bacteriological Reviews 35(2):171-205.
Xu, H., D. Vavilin, C. Funk, and W. Vermaas. "Multiple Deletions of Small-Cab-like Proteins.