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Bromothymol Blue, Sodium Salt
Product ID: B1714


Description: Bromothymol blue (BTB) is often used in electrophoresis to estimate the mobility of the electrophoretic front. BTB is also a biological stain and pH indicator; yellow at pH 6.0, green at pH 7.0, and blue at pH 7.6.

Synonyms: BTB; 3',3''-dibromothymolsulfonephthalein sodium salt; Bromothymol Blue sodium salt; Bromothymol Sulfone Phthalein

Solubility Water
Physical Form Solid
Storage Temp. Room Temperature
UPC / SKU B1714
CAS NUMBER 34722-90-2
Formula Weight 646.37
Formula C27H27Br2O5SNa
Synonyms BTB; 3′,3′′-dibromothymolsulfonephthalein sodium salt; Bromthymol Blue sodium salt; Bromothymol Sulfone Phthalein
Storage Temp. Room Temperature
Tariff Code 2914.70.4000
Risk Info (R) 38-41
Safety Info (S) 22-36/37/39
No information available

B1714 Bromothymol Blue, Sodium Salt

Synonyms: Bromothymol Sulfone Phthalein, BTB, 3′,3″-Dibromothymolsulfonphthalein sodium salt
CAS: 34722-90-2
Formula: C27H27Br2O5SNa
Mol. Weight: 646.37
Form: Powder
Appearance: Orange to Black
Solubility: Soluble in Water
Application: Phytopathology, Seed Testing, Microbiology
Typical Working Concentration: Varies with application, should be determined by end user.
Storage Temp: Room Temperature

Application Notes
Generally used as a biological stain and pH indicator. Bromothymol Blue (BTB) is yellow at a pH of 6.0 and gradually turns to blue as the pH rises to 7.6. It can be incorporated into a medium to observe color change, such as the inclusion of BTB in MSP agar (ISTA, 2011) or any other microbiology medium where a pH change can be indicative of bacterial/fungal growth.

ISTA (2011) Annexe to Chapter 7: Seed Health Methods. Kawanishi T, T. Shiraishi, Y. Okano, K. Sugawara, M. Hashimoto, K Maejima, K Komatsu, S Kakizawa, Y Yamaji, H Hamamoto, K Oshima, S Namba (2011) New Detection Systems of Bacteria Using Highly Selective Media Designed by SMART: Selective Medium-Design Algorithm Restricted by Two Constraints. PLoS ONE Volume 6, Issue 1.