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BM-2 Terrestrial Orchid Medium without Agar
Product ID: B470


Description: Contains the macro-and micronutrients, vitamins, sucrose fructose, agar, and supplements required to culture terrestrial orchids.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 21.22
Other Notes Plant Tissue Culture Tested
UPC / SKU B470
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
B210 Boric Acid 10
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.25
F263 Ferrous Sulfate, Heptahydrate 27.85
M150 Magnesium Sulfate, Anhydrous 100
M250 Manganese Sulfate 25
P846 Potassium Phosphate, Monobasic, Anhydrous 300
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 10
B800 6-Benzylaminopurine (6-BA) 0.2
B140 D-Biotin 0.05
C184 Casein, Enzymatic Hydrolysate 500
F430 Folic Acid 0.5
G229 L-Glutamine 100
G503 Glycine 2
I703 myo-Inositol 100
N765 Nicotinic Acid 5
P866 Pyridoxine HCl 0.5
S391 D-Sucrose 20,000
T390 Thiamine HCl 0.5

B470 BM-2 Terrestrial Orchid Medium w/out Agar

Form: Powder
Appearance: White to Cream
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 21.22 g/L
Storage Temp: 2-6° C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Same formulation as B141 with 0.2 mg/L BA
Unadjusted pH = 5.0 – 6.0
BM-2 differs from BM Medium in that it (BM-2) has no inorganic nitrogen and it contains hydrolyzed casein, L-glutamine and BA.
Formula (mg/L)
Boric Acid 10
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O 37.25
Ferrous Sulfate.7H2O 27.85
Magnesium Sulfate, Anhydrous 100
Manganese Sulfate.H2O 25
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Phosphate, Monobasic 300
Zinc Sulfate.7H2O 10
6-Benzylaminopurine (BA) 0.2
D-Biotin 0.05
Casein, Enzymatic Hydrolysate 500
Folic Acid 0.5
L-Glutamine 100
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 5
Pyridoxine.HCl 0.5
Sucrose 20,000
Thiamine.HCl 0.5

Application Notes
Plant Tissue Culture Tested
Well suited for the terrestrial orchids Paphiopedilum and Phragmipedium.

According to "The Orchid Seedbank Project" this was originally cited by Van Waes, 1984. In vitro studie van de kiemingsfysiologie van Westeuropese orchideeėn. Thesis. Rijkuniversiteit Gent.