Blaydes Basal Medium Product ID: B514
Introduction
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
31.86 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
B514 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
1000 |
| B210 |
Boric Acid |
1.6 |
| C180 |
Calcium Nitrate |
241.1 |
| E410 |
EDTA, Disodium Salt |
74.5 |
| F263 |
Ferrous Sulfate, Heptahydrate |
55.7 |
| M150 |
Magnesium Sulfate, Anhydrous |
17.1 |
| M250 |
Manganese Sulfate |
4.4 |
| P704 |
Potassium Chloride |
100 |
| P840 |
Potassium Iodide |
0.8 |
| P100 |
Potassium Nitrate |
65 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
300 |
| Z101 |
Zinc Sulfate, Heptahydrate |
1.5 |
| G503 |
Glycine |
2.0 |
| S391 |
D-Sucrose |
30,000 |
| T390 |
Thiamine HCl |
0.1 |
B514 Blaydes Modified Basal Medium
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Off-white to yellow |
| Application: |
Plant Tissue Culture |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
31.86 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients, sucrose, and thiamine as described by Blaydes (1966). Plant Tissue Culture Tested. |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
1000 |
| Boric Acid |
1.6 |
| Calcium Nitrate |
241.1 |
| Na2EDTA.2H2O |
74.5 |
| Ferrous Sulfate.7H2O |
55.7 |
| Magnesium Sulfate, Anhydrous |
17.1 |
| Manganese Sulfate.H2O |
4.4 |
| Potassium Chloride |
65 |
| Potassium Iodide |
0.8 |
| Potassium Nitrate |
100 |
| Potassium Phosphate, Monobasic |
300 |
| Zinc Sulfate.7H2O |
1.5 |
| Glycine (Free Base) |
2 |
| Sucrose |
30,000 |
| Thiamine.HCl |
0.1 |
Application Notes
Plant species: Alfalfa
Callus initiation was achieved when the basal medium was supplemented with 1.9 mg/L Kinetin and 8.0 – 19.0 mg/L 2,4-D. Regeneration was achieved when the Kinetin and 2,4-D were omitted and the basal medium was supplemented with 100 mg/L myo-Inositol and 2,000 mg/L yeast extract
References
Blaydes, OF. 1966. Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant 19: 748-753.
|
|
