Introduction
Description: A carbon source and substrate for ß-glucosidase.
Can be used in mD5A Medium (Prod. # M5516) for the selection of Xanthomonas hortorum. Also used in Cefazoline-Cellobiose-Methionine (CCM) medium as a carbon source for Xanthomonas axonopodis pv. vignicola.See Product Information Sheet for details.Synonyms: 4-O-ß-D-Glucopyranosyl-D-glucose
| Solubility |
Water |
| Use With |
M5516 - mD5A Medium |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| UPC / SKU |
C1842 |
| CAS NUMBER |
528-50-7 |
| Formula Weight |
342.3 |
| Formula |
C12H22O11 |
| Synonyms |
4-O-ß-D-Glucopyranosyl-D-glucose |
| Storage Temp. |
Room Temperature |
| Tariff Code |
2940.00.6000 |
No information available
C1842 Cellobiose
| Synonyms: |
4-O-β-D-Glucopyranosyl-D-glucose |
| CAS: |
528-50-7 |
| Formula: |
C12H22O11 |
| Mol. Weight: |
342.30 |
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Off-White |
| Application: |
Microbiology, Seed Testing, Phytopathology |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
Varies with application, should be determined by end user. |
| Storage Temp: |
Room Temperature |
| Product# |
Product Name |
Amount/L |
| Product # |
Product Name |
Amount/L |
| P705 |
Dipotassium Phosphate, (K2HPO4) |
1.34 g/L |
| P846 |
Monopotassium Phosphate (KH2PO4) |
0.4 g/L |
| M150 |
Magnesium Sulfate (Anhydrous) (MgSO4) |
0.3 g/L |
| B210 |
Boric Acid (H3BO3) |
0.2 g/L |
| A109 |
Ammonium Chloride |
1.0 g/L |
| C1842 |
Cellobiose |
10.0 g/L |
| M539 |
Methionine |
1.0 g/L |
| A111/A296 |
Agar |
14.0 g/L |
| C1989/C1796 |
*Cycloheximide Powder/ Solution (100 mg/mL) |
0.2 g/L or 2.0 mL/L |
| N/A |
*Cefazoline |
0.010 g/L |
| |
Adjust to pH 7.2 Add aseptically after autoclaving medium and allowing to cool. |
|
Application Notes
Cellobiose is used in microbiological and phytopathological media as a carbon source for selection of specific microbes. It is used in multiple selection media, including mD5A Medium (Prod # M5516) and CCM medium. The table below is an example of the use of cellobiose as a carbon source in a selection medium.
The semi-selective medium designated as Cefazoline-Cellobiose-Methionine (CCM) medium is intended to select for Xanthomonas axonopodis pv. vignicola and contains the following components per liter (Wydra et al., 2004):
References
Wydra K, G Khatri-Chhetri, A Mavridis, R Sikirou, K Rudolph (2004) A diagnostic medium for the semi-selective
isolation and enumeration of Xanthomonas axonopodis pv. vignicola. European Journal of Plant Pathology,110(10) pp. 991-1001.
