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Calcofluor White M2R
Product ID: C1933


Description: Bromothymol blue (BTB) is often used in electrophoresis to estimate the mobility of the electrophoretic front.

BTB is also a biological stain and pH indicator; yellow at pH 6.0, green at pH 7.0, and blue at pH 7.6.

Synonyms: BTB; 3',3''-dibromothymolsulfonephthalein sodium salt; Bromothymol Blue sodium salt; Bromothymol Sulfone Phthalein.

Solubility Water
Physical Form Solid
Storage Temp. Room Temperature
UPC / SKU C1933
CAS NUMBER 4193- 55-9
Formula Weight 960.95
Formula C40H42N12O10S2
Synonyms Calcofluor White ST
Storage Temp. Room Temperature
No information available

C1933 Calcofluor White M2R

Synonyms: Calcofluor White ST; Fluorescent Brightner 28 Disodium Salt; Cellufluor
CAS: 4193-55-9
Formula: C40H42N12O10S2Na2
Mol. Weight: 960.95
Form: Powder
Appearance: Yellow to Tan
Application: Fluorescent Stain, Histology
Solubility: Water
Typical Working Concentration: Varies with application, should be determined by end user.
Storage Temp: Room Temperature

Application Notes
Calcofluor White is a fluorescent stain that has been used to indicate cell wall biosynthesis for plant and fungal cells (Nagata & Takebe, 1970). Calcofluor White fluoresces when it hydrogen bonds with beta-1,4 and beta-1,3 glucosidic linkages, which are primary components of cellulose and chitin in cell walls. These types of fluorescent dyes are known as optical brightening agents because they absorb near-UV light (broad region lambda max = 315- 415 nm) and emit in the blue-green wavelength region (broad region lambda max = 430-550 nm). It can also be used for the staining of fungi and as a viability stain.

Nagata T. and Takebe I. (1970) Cell well regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta Vol. 92 pg. 301-308.