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PhytoTechnology Laboratories

Carrot Shoot Development Basal Medium
Product ID: C222


Description: Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate shoots from carrot callus.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 3.21
Other Notes Plant Tissue Culture Tested
UPC / SKU C222
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A305 Ammonium Sulfate 134
B210 Boric Acid 3.0
C266 Calcium Chloride, Anhydrous 113.24
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 122.09
M250 Manganese Sulfate 10
P840 Potassium Iodide 0.75
P100 Potassium Nitrate 2500
M651 Sodium Molybdate (VI), Dihydrate 0.25
S515 Sodium Phosphate, Monobasic 150
Z101 Zinc Sulfate, Heptahydrate 2.0
I703 myo-Inositol 100
K750 Kinetin 0.2
N765 Nicotinic Acid 1.0
P866 Pyridoxine HCl 1.0
T390 Thiamine HCl 10

C222 Carrot Shoot Development Basal Medium

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 3.21g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate carrot callus from root tissue. Requires the addition of sucrose (e.g., 30 g/L) and a gelling agent (e.g., 6-8 g/L agar) pH = 3.5 – 4.5; should be adjusted to pH 5.6 – 5.8
Formula (mg/L)
Ammonium Sulfate 134
Boric Acid 3
Calcium Chloride, Anhydrous 113.24
Cobalt Chloride·6H2O 0.025
Cupric Sulfate·5H2O 0.025
Na2 EDTA·2H2O 37.3
Ferrous Sulfate·7H2O 27.8
Magnesium Sulfate, Anhydrous 122.09
Manganese Sulfate·H2O 10
Molybdic Acid (Sodium Salt)·2H2O 0.25
Potassium Iodide 0.75
Potassium Nitrate 2500
Sodium Phosphate Monobasic 150
Zinc Sulfate·7H2O 2
2,4-Dichlorophenoxyacetic Acid 1
myo-Inositol 100
Nicotinic Acid (Free Acid) 1
Pyridoxine·HCl 1
Thiamine·HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Carrot (Daucus carota)