Carrot Shoot Development Basal Medium Product ID: C222
Introduction
Description: Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate shoots from carrot callus.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
3.21 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
C222 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A305 |
Ammonium Sulfate |
134 |
| B210 |
Boric Acid |
3.0 |
| C266 |
Calcium Chloride, Anhydrous |
113.24 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.26 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
122.09 |
| M250 |
Manganese Sulfate |
10 |
| P840 |
Potassium Iodide |
0.75 |
| P100 |
Potassium Nitrate |
2500 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| S515 |
Sodium Phosphate, Monobasic |
150 |
| Z101 |
Zinc Sulfate, Heptahydrate |
2.0 |
| I703 |
myo-Inositol |
100 |
| K750 |
Kinetin |
0.2 |
| N765 |
Nicotinic Acid |
1.0 |
| P866 |
Pyridoxine HCl |
1.0 |
| T390 |
Thiamine HCl |
10 |
C222 Carrot Shoot Development Basal Medium
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
3.21g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate carrot callus from root tissue. Requires the addition of sucrose (e.g., 30 g/L) and a gelling agent (e.g., 6-8 g/L agar) pH = 3.5 – 4.5; should be adjusted to pH 5.6 – 5.8 |
| Formula (mg/L) |
|---|
| Ammonium Sulfate |
134 |
| Boric Acid |
3 |
| Calcium Chloride, Anhydrous |
113.24 |
| Cobalt Chloride·6H2O |
0.025 |
| Cupric Sulfate·5H2O |
0.025 |
| Na2 EDTA·2H2O |
37.3 |
| Ferrous Sulfate·7H2O |
27.8 |
| Magnesium Sulfate, Anhydrous |
122.09 |
| Manganese Sulfate·H2O |
10 |
| Molybdic Acid (Sodium Salt)·2H2O |
0.25 |
| Potassium Iodide |
0.75 |
| Potassium Nitrate |
2500 |
| Sodium Phosphate Monobasic |
150 |
| Zinc Sulfate·7H2O |
2 |
| 2,4-Dichlorophenoxyacetic Acid |
1 |
| myo-Inositol |
100 |
| Nicotinic Acid (Free Acid) |
1 |
| Pyridoxine·HCl |
1 |
| Thiamine·HCl |
10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Carrot (Daucus carota)
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