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Charcoal, Activated
Product ID: C325


Description: Activated charcoal absorbs and acts as a reservoir for growth inhibitory secondary products given off by plant tissue. Acid washed.
Synonyms: Carbon Black; Activated Carbon.

Solubility Water
Physical Form Solid
Storage Temp. Room Temperature
Other Notes Plant Tissue Culture Tested
UPC / SKU C325
CAS NUMBER 7440-44-0
Formula Weight 12.01
Formula C
Synonyms Carbon Black; Activated Carbon
Storage Temp. Room Temperature
Tariff Code 3802.10.0000
Risk Info (R) 20-36/37/38
Safety Info (S) 22-26-36/37/39
No information available

C325 Charcoal, Activated, Acid Washed

Synonym: Carbon Black; Activated Carbon
CAS: 7440-44-0
Formula: C
Molecular Wt:12.01

Form: Powder
Appearance: Black Fine Powder
Application: Orchid Media Supplement
Solubility: Insoluble - Forms Suspension with Water
Typical Working Concentration: 1 to 5 grams per liter
Storage Temp: Room Temperature
Other Notes: Plant Tissue Culture Tested

Application Notes
Activated charcoal (AC) works by ad sorbing toxic substances (e.g., phenols , inorganic compounds , and other organic impurities ) secreted by plant tissues or present in media ingredient that would otherwise affect growth. Due to its ability to a dsorb many impurities, usages of AC in culture media had been reported to improve and/or stimulate growth, organogenesis and embryogenesis for many plant species.2.3

Typical concentration of AC ranges from 1 to 5 g/L which has been reported to help reduce polyphenol exudation in immature embryos of Sorghum bicolour ; however, a low concentration of 0.5 g/L has been reported to help reduce polyphenol from Aristolochia indica .3

For orchid tissue culture, AC is also com monly used in orchid media to adsorb phenolics compounds secreted by wounded plant tissues to prev ent darkening of media which would inhibit plant growth. Seedlings of Phalaenopsis when cultured on media supplemented with AC at 0.5 g/L ha ve shown significant shoot and root development; while at 3 g/L of AC help ed increase root development of Cymbidium and Dendrobium .4

While m any a dvantages has been reported for the use of activated charcoal , a report of AC interference with growth hormones has been report in which it a dsorbed the required growth hormones for the growth development of callus growth of Nicotiana tabacum cv. Wisconsin 38.5

Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used

1. Merck 13, 1818
2. George G. 1993. Plant Pr opagation by Tissue Culture, Part 1: The Technology. England: Exegetics Limited, 574 pp.
3. Thomas, T. Dennis. 2008. The Role of Activated Charcoal in Plant Tissue Culture. Biotechnology Advances. 26:618-631