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Copper (II) Sulfate Solution (4% w/v)
Product ID: C466


Description: Copper (II) Sulfate is used as a copper source in the bicinchonic acid assay for protein

Grade ACS Reagent
Miscibility Water
Physical Form Liquid
Sterility Sterile Filtered
Storage Temp. Room Temperature
CAS NUMBER 7758-99-8
Formula Weight 249.68
Formula CuSO45H2O
Manufactured from C375
Storage Temp. Room Temperature
Tariff Code 2833.25.0000
Risk Info (R) 50/53
Safety Info (S) 61
No information available

C466 Copper (II) Sulfate Solution (4%)

Synonyms: Copper(II) Sulfate Pentahydrate; Cupric Sulfate
CAS: 7758-99-8
Formula: CuSO4•5H2O
Mol. Weight: 249.68
Form: Liquid
Appearance: Blue, Clear
Application: Plant Micronutrient, Fungicide, Herbicide, Algicide
Solubility: Miscible with Water
Storage Temp: Room Temperature
Other Notes: Plant Tissue Culture Tested

Application Notes
Used as a micronutrient in plant tissue culture. Can also be used as a fungicide, although some fungi are capable of persisting through elevated levels of copper ions (Johnson, 1935). As an herbicide, it can be used to control the growth of aquatic plants, as well as algae (Newbold, 1975; Robson et al, 1976). It also inhibits the growth of bacteria, such as Escherichia coli (Tetaz & Luke, 1983).

It is used in Fehling’s Solution and Benedict’s Solution to test for reducing sugars. Reducing sugars will cause the blue color of the copper in solution to change to an insoluble red solution of copper(I) oxide. Can also be used in the Biuret reagent to test for proteins.

Merck 13, 2682 Gadd, G. M., & Griffiths, A. J. (1977). Microorganisms and heavy metal toxicity. Microbial Ecology, 4(4), 303-317.
Johnson, GF (1935) "The Early History of Copper Fungicides". Agricultural History 9(2): 67–79. Le Jeune, A. H., Charpin, M., Deluchat, V., Briand, J. F., Lenain, J. F., Baudu, M., & Amblard, C. (2006). Effect of copper sulphate treatment on natural phytoplanktonic communities. Aquatic toxicology, 80(3), 267-280.
Newbold, C (1975) Herbicides in aquatic systems. Biological Conservation 7(2):97-118. Robson, T. O., Fowler, M. C., & Barrett, P. R. (1976). Effect of some herbicides on freshwater algae. Pesticide Science, 7(4), 391-402.
Sadler, W. R., & Trudinger, P. A. (1967). The inhibition of microorganisms by heavy metals. Mineralium Deposita, 2(3), 158-168.
Tetaz, T. J., & Luke, R. K. (1983). Plasmid-controlled resistance to copper in Escherichia coli. Journal of bacteriology, 154(3), 1263-1268