Introduction
Description: Contains macro- and micronutrients as described by Gupta & Durzan (1985).
This medium was developed to promote shoot proliferation. This formulation has approximately ¼ the concentration of NH4NO3 and KNO3 compared to MS. Additional Ca+2 and NO3- ions are supplied by Ca(NO3)2.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
1.64 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
D146 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
400 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
64.14 |
| C180 |
Calcium Nitrate |
386.31 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.25 |
| E410 |
EDTA, Disodium Salt |
37.3 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
| M250 |
Manganese Sulfate |
22.3 |
| N478 |
Nickel Chloride Hexahydrate |
0.025 |
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
340 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
170 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
D146 DCR Basal Salt Mixture
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
1.64g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Chu et al. (1975). pH = 3.5 – 4.5 |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
400 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
64.14 |
| Calcium Nitrate |
386.31 |
| Cobalt Chloride·6H2O |
0.025 |
| Cupric Sulfate·5H2O |
0.25 |
| Na2 EDTA·2H2O |
37.3 |
| Ferrous Sulfate·7H2O |
27.8 |
| Magnesium Sulfate, Anhydrous |
180.7 |
| Manganese Sulfate·H2O |
22.3 |
| Molybdic Acid (Sodium Salt)·2H2O |
0.25 |
| Nickel Chloride·6H2O |
0.025 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
340 |
| Potassium Phosphate, Monobasic |
170 |
| Zinc Sulfate·7H2O |
8.6 |
Application Notes
Plant Tissue Culture Tested
Plant Species: Douglas Fir (Pseudotsuga spp.) and Sugar Pine (Pinus lambertiana)
This medium was developed to promote shoot proliferation. This formulation has approximately ¼ the concentration of NH4NO3 and KNO3 compared to MS. Additional Ca+2 and NO3 - ions are supplied by Ca(NO3)2.
References
Gupta, PK and KJ Durzan. 1985. Shoot multiplication from mature Douglas-fir and sugar pine. Plant Cell Reports 4:177-179.
