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DKW Basal Medium with 30 g/L Sucrose
Product ID: D189


Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 35.22
Other Notes Plant Tissue Culture Tested
UPC / SKU D189
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 1416
B210 Boric Acid 4.8
C266 Calcium Chloride, Anhydrous 112.5
C180 Calcium Nitrate 1367
C375 Cupric Sulfate, Pentahydrate 0.25
E410 EDTA, Disodium Salt 45.5
F263 Ferrous Sulfate, Heptahydrate 33.8
M150 Magnesium Sulfate, Anhydrous 361.49
M250 Manganese Sulfate 33.5
N458 Nickel Sulfate Hexahydrate 0.005
P846 Potassium Phosphate, Monobasic, Anhydrous 265
P854 Potassium Sulfate, Anhydrous 1559
M651 Sodium Molybdate (VI), Dihydrate 0.39
Z897 Zinc Nitrate, Hexahydrate 17
S391 D-Sucrose 30,000

D189 DKW Basal Medium

Synonym: DKW Basal Salts w/ Sucrose;
Driver and Kuniyuki Walnut medium

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 35.22 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Driver and Kuniyuki (1984) and McGranahan, et al. (1987). Also contains sucrose. pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate 1416
Boric Acid 4.8
 Calcium Chloride, Anhydrous 112.5
 Calcium Nitrate 1367
Cupric Sulfateˇ5H2O 0.25
Na2 EDTAˇ2H2O 45.4
 Ferrous Sulfateˇ7H2O 33.8
Magnesium Sulfate, Anhydrous 361.49
Manganese SulfateˇH2O 33.5
 Molybdic Acid (Sodium Salt)ˇ2H2O  0.39
 Nickel Sulfateˇ6H2O 0.005
 Potassium Phosphate, Monobasic  265
 Potassium Sulfate 1559
 Zinc Nitrateˇ6H2O  17
 Sucrose 30,000

Application Notes
Plant species: Northern California Walnut (Juglans hindsii). This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 µM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse

Driver, J.A. and A.H. Kuniyuki. 1984. In vitro propagation of Paradox walnut rootstock. HortScience 19:507–509. McGranahan, GH, et al. 1987. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.