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De Greef & Jacobs Basal Medium
Product ID: D2206


Description: Contains the macro- and micronutrients and vitamins as described by De Greef and Jacobs (1979).

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 3.91
Other Notes Plant Tissue Culture Tested
UPC / SKU D2206
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A305 Ammonium Sulfate 400
B210 Boric Acid 10.62
C266 Calcium Chloride, Anhydrous 226.5
C350 Cobalt Chloride Hexahydrate 0.0025
C375 Cupric Sulfate, Pentahydrate 0.0025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 244.33
M250 Manganese Sulfate 1.68
P704 Potassium Chloride 600
P840 Potassium Iodide 1.58
P100 Potassium Nitrate 2000
M651 Sodium Molybdate (VI), Dihydrate 0.0025
S515 Sodium Phosphate, Monobasic 250
Z101 Zinc Sulfate, Heptahydrate 1.06
I703 myo-Inositol 100
N765 Nicotinic Acid 1.0
P866 Pyridoxine HCl 1.0
T390 Thiamine HCl 10.0


Form: Powder
Appearance: White to Light Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 3.91g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by De Greef & Jacobs (1979). Plant Tissue Culture Tested Unadjusted pH (approximately): 4.0 – 5.0
Formula (mg/L)
Ammonium Sulfate 400.0
 Potassium Iodide 1.58
 Boric Acid 10.62
 Potassium Nitrate 2,000
 Calcium Chloride, Anhydrous  226.5
 Sodium Phosphate, Monobasic 250.0
Cobalt Chloride 0.0025
Sodium Molybdate 0.0025
 Cupric Sulfate  0.0025
 Zinc Sulfate 7H2O 1.06
 Ferrous Sulfate 7H2O 27.8
 myo-Inositol  100.0
 Magnesium Sulfate Anhydrous 244.33
 Nicotinic Acid  1.0
 Manganese Sulfate H2O 1.68
Pyridoxine HCl 1.0
 Na2 EDTA 2H2O 37.26
 Thiamine HCl 10.0
 Potassium Chloride 600.0

Application Notes
Originally used in the tissue culture of sugar beets.

De Greef W, Jacobs M. 1979. In vitro culture of sugar beet: description of a cell line with high regeneration capacity. Plant Science Letters 17, 55-61.