Introduction
Description: DNA Denaturing Solution
| Miscibility |
Water |
| Physical Form |
Liquid |
| Sterility |
Sterile Filtered |
| Storage Temp. |
Room Temperature |
| UPC/SKU |
D548 |
| Storage Temp. |
Room Temperature |
No information available
D548 DNA Denaturing Solution
| Properties |
|---|
| Form: |
Liquid |
| Appearance: |
Colorless, Clear |
| Application: |
Molecular Biology |
| Solubility: |
Miscible in Water |
| Typical Working Concentration: |
Varies according to application |
| Storage Temp: |
Room Temperature |
| Other Notes: |
Sterile Filtered, RNAse & DNAse free |
| Properties |
|---|
| Sodium Hydroxide |
20000 |
| Sodium Chloride |
87660 |
Application Notes
This solution is used to denature DNA prior to transfer from electrophoresis gel to nitrocelllose or nylon membrane.
References
Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual, 2nd Edition. Cold Spring Harbor Laboratory Press.
Ausubel, F.M. et al., eds. (1993) Current Protocols in Molecular Biology. Greene Publishing Associates, Inc., in association with John Wiley & Sons, Inc.
Davis, L.G., Dibner, M.D. & Battey, J.F. (1986) Basic Methods in Molecular Biology. Elsevier Science Publishing Company, Inc.
