Introduction
Description: A biological stain used to test light-colored seeds for mechanical damage.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| UPC / SKU |
F3178 |
| CAS NUMBER |
2353-45-9 |
| Formula Weight |
808.85 |
| Formula |
C37H34N2O10S3Na2 |
| Synonyms |
Fast Green (FCF); ethyl-[4-[[4-[ethyl-[(3-sulfophenyl)methyl]amino]phenyl]-(4-hydroxy-2-sulfophenyl)methylidene]-1-cyclohexa-2,5-dienylidene]-[(3-sulfophenyl)methyl]azanium; C.I.# 42053 |
| Storage Temp. |
Room Temperature |
| Tariff Code |
3204.90.0000 |
| Risk Info (R) |
68 |
| Safety Info (S) |
36/37 |
No information available
F3178 Fast Green FCF
| Synonyms: |
Green 1724, Solid Green FCF, C.I. 42053 |
| CAS: |
2353-45-9 |
| Formula: |
C37H34N2O10S3Na2 |
| Mol. Weight: |
808.85 |
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Dark Green to Brown |
| Application: |
Seed Testing |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
Varies with application, should be determined by end user. |
| Storage Temp: |
Room Temperature |
Application Notes
The Fast Green stain is used in seed testing to determine the pericarp integrity (Koehler 1957). Koehler used a 0.1% solution of fast green applied to maize seeds to stain the seeds for seed coat damage (1957). After a short soak of 15-30 seconds, the seeds are washed and analyzed for damage.
Fast green is also commonly used to stain plant tissues in conjunction with safranin O.
References
Koehler B (1957) Pericarp injuries in seed corn. Bull. No. 617:1–72. Illinois Agric. Exp. Stn., Urbana, IL.
