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Fast Green
Product ID: F3178


Description: A biological stain used to test light-colored seeds for mechanical damage.

Solubility Water
Physical Form Solid
Storage Temp. Room Temperature
UPC / SKU F3178
CAS NUMBER 2353-45-9
Formula Weight 808.85
Formula C37H34N2O10S3Na2
Synonyms Fast Green (FCF); ethyl-[4-[[4-[ethyl-[(3-sulfophenyl)methyl]amino]phenyl]-(4-hydroxy-2-sulfophenyl)methylidene]-1-cyclohexa-2,5-dienylidene]-[(3-sulfophenyl)methyl]azanium; C.I.# 42053
Storage Temp. Room Temperature
Tariff Code 3204.90.0000
Risk Info (R) 68
Safety Info (S) 36/37
No information available

F3178 Fast Green FCF

Synonyms: Green 1724, Solid Green FCF, C.I. 42053
CAS: 2353-45-9
Formula: C37H34N2O10S3Na2
Mol. Weight: 808.85
Form: Powder
Appearance: Dark Green to Brown
Application: Seed Testing
Solubility: Soluble in Water
Typical Working Concentration: Varies with application, should be determined by end user.
Storage Temp: Room Temperature

Application Notes
The Fast Green stain is used in seed testing to determine the pericarp integrity (Koehler 1957). Koehler used a 0.1% solution of fast green applied to maize seeds to stain the seeds for seed coat damage (1957). After a short soak of 15-30 seconds, the seeds are washed and analyzed for damage.

Fast green is also commonly used to stain plant tissues in conjunction with safranin O.

Koehler B (1957) Pericarp injuries in seed corn. Bull. No. 617:1–72. Illinois Agric. Exp. Stn., Urbana, IL.