  Guaiacol
| ||||||||||||||||||||||||||||||||||||||||||||||||||
| Solubility | Methanol |
| Miscibility | Methanol |
| Physical Form | Solid or Liquid (26.1°C melting point) |
| Storage Temp. | Room Temperature |
| UPC / SKU | G3574 |
| CAS NUMBER | 90-05-1 |
| Formula Weight | 124.14 |
| Formula | C7H8O2 |
| Synonyms | 2-methoxyphenol; o-methoxyphenol |
| Storage Temp. | Room Temperature |
| Tariff Code | 3822.00.5090 |
| Risk Info (R) | 22-36/38 |
| Safety Info (S) | 26 |
G3574 Guaiacol
| Synonyms: | 2-methoxyphenol |
| CAS: | 90-05-1 |
| Formula: | C7H8O2 |
| Mol. Weight: | 124.14 |
| Properties | |
|---|---|
| Form: | Liquid @ Room Temperature |
| Appearance: | Clear, Colorless to Slight Yellow or Pink |
| Solubility: | Miscible with Methanol |
| Application: | Colorimetric Assay, Seed Testing |
| Storage Temp: | Room Temperature |
| Typical Working Concentration: | Varies with application, should be determined by end user. |
| Other Notes: | Light & Air Sensitive |
Application Notes
Guaiacol is commonly used in colorimetric assays to measure peroxidase enzyme activity. Initially colorless, guaiacol undergoes polymerization in the presence of a peroxidase and H2O2 to form a brown compound known as tetraguaiacol which can be measured spectrophotometrically at roughly 470 nm (Johannes, 2006; Doerge, 1997; Balešević-Tubić, 2011).
Guaiacol has also been documented to detect Helminthosporium oryzae and Trichoconis padwickii in rice seeds when incorporated into an agar medium with streptomycin (prod # S739 or S7739) by staining the surrounding medium red in the presence of the fungus (Kulik, 1975). Medium components according to Kulik (1975): 0.125g guaiacol, 5g agar & 0.5g streptomycin (added after autoclaving) per liter of water. For more information on this method, see Kulik reference below.
In soybean seed testing, guaiacol separates different cultivars based on high and low seed coat peroxidase activity. The level of peroxidase activity of the seed coat is based on the color change of the guaiacol (Schmitz, 1997).
References
Anto K.B., K.M. Jayaram and Nabeesa Salim (2010) Studies on thermo-tolerance, germination behaviour and activity of guaiacol peroxidase enzyme in pea (Pisum sativum L.) and soybean [Glycine max (L.)Merr.] seeds during early stages of germination. International Journal of Plant Sciences, 5(2): 547-552.
Balešević-Tubić, S., Mladen Tatić, Vuk Đorđević, Zorica Nikolić, Jonel Subić and Vojin Đukić (2011) Changes in soybean seeds as affected by accelerated and natural aging. Romanian Biotechnological Letters,16(6). Brücher, H. (1950) A new biochemical rapid method for determination of germinating power in cereals. Sveriges Utsadesforenings Tidskrift, 60: 210-214
Doerge D.R., Rao L. Divi, Mona I. Churchwell (1997) Identification of the Colored Guaiacol Oxidation Product Produced by Peroxidases. Analytical Biochemistry, 250(1): Pp. 10–17.
Johannes T.W., R. Woodyer, and H. Zhao (2006) Enzyme Assays: High-throughput Screening, Genetic Selection and Fingerprinting. Edited by Jean-Louis Reymond. Wiley Publishing, Weinheim. Pp. 77-93.
Kulik, M (1975) Comparison of Blotters and Guaiacol Agar for Detection of Helminthosporium oryzae and Trichoconis padwickii in Rice Seeds. Phytopathology, 45: pp.1325-26.
Schmitz N., Robert van Huystee, Mark Gijzen. (1997) Characterization of anionic soybean (Glycine max) seed coat peroxidase. Canadian Journal of Botany, 75(8): 1336-1341, 10.1139/b97-845
