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Gamborg B-5 Basal Medium
Product ID: G398


Description: Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
This medium was developed for the initiation and growth of soybean cell suspensions. This medium contains no Ammonium Nitrate; it does contain Ammonium Sulfate and increased levels of Potassium Nitrate. Concentrations of NH4+ over 2 mM inhibited cell growth.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 3.21
Other Notes Plant Tissue Culture Tested
UPC / SKU G398
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A305 Ammonium Sulfate 134
B210 Boric Acid 3.0
C266 Calcium Chloride, Anhydrous 113.24
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 122.09
M250 Manganese Sulfate 10
P840 Potassium Iodide 0.75
P100 Potassium Nitrate 2500
M651 Sodium Molybdate (VI), Dihydrate 0.25
S515 Sodium Phosphate, Monobasic 150
Z101 Zinc Sulfate, Heptahydrate 2.0
I703 myo-Inositol 100
N765 Nicotinic Acid 1.0
P866 Pyridoxine HCl 1.0
T390 Thiamine HCl 10

G398 Gamborg B-5 Basal Medium

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 3.21g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Gamborg, et al (1968). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Sulfate  134
Boric Acid 3
Calcium Chloride, Anhydrous  113.24
Cobalt Chloride·6H2O  0.025
Cupric Sulfate·5H2O  0.025
Na2 EDTA·2H2O  37.26
Ferrous Sulfate·7H2O  27.8
Magnesium Sulfate, Anhydrous  122.09
Manganese Sulfate·H2O  10
Molybdic Acid (Sodium Salt)·2H2O  0.25
Potassium Iodide  0.75
Potassium Nitrate  2500
Sodium Phosphate Monobasic  150
Zinc Sulfate·7H2O 2
myo-Inositol  100
Nicotinic Acid (Free Acid)  1
Pyridoxine·HCl  1
Thiamine·HCl  10

Application Notes
Plant Species: Soybean (Glycine max) This medium was developed for the initiation and growth of soybean cell suspensions.
This medium contains no ammonium nitrate; it does contain ammonium sulfate and increased levels of potassium nitrate.Concentrations of NH4 + over 2 mM inhibited cell growth. Thiamine, an essential vitamin, is at a concentration 10x that of Linsmaier & Skoog Medium.

Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.