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Gibberellic Acid
Product ID: G500

Introduction

Description: Gibberellic acid promotes internode elongation & breaks certain dormancies in seeds.
Synonyms: GA3; Gibberellin A3

Solubility KOH, EtOH
Physical Form Solid
Solutions Available G362
Storage Temp. 2 to 6 °C
Other Notes Plant Tissue Culture Tested
UPC / SKU G500
CAS NUMBER 77-06-5
Formula Weight 346.37
Formula C19H22O6
Synonyms GA3; Gibberellin A3
Storage Temp. 2 to 6 °C
Tariff Code 2932.20.5050
Risk Info (R) 36/37/38
Safety Info (S) 26-36
No information available

G500 Gibberellic Acid

Synonyms: GA3; Gibberellin A3
CAS: 77-06-5
Formula: C19H22O6
Mol. Weight: 346.41
Properties
Form: Powder
Appearance: White to Cream
Application: Plant Growth Regulator, Seed Testing
Solubility: Ethanol (EtOH), 1N KOH
Typical Working Concentration: 0.01 – 5.0 mg/L, varies depending on application, should be determined by end user.
Storage Temp: 2 to 6˚ C
Other Notes: Plant Tissue Culture Tested; For Research Use Only

Application Notes
Gibberellins are known to promote shoot (internode) elongation and break certain dormancies in seeds. They also can induce flowering in some plant species.2

According to the CFIA (Canadian Food Inspection Agency), gibberellic acid can be used in a concentration range from 0.02-0.10% to break seed dormancy. The CFIA (2011) recommends that if a concentration of 0.08% or higher is to be used, the gibberellic acid should be dissolved in a phosphate buffer consisting of the following per liter of water:

1.78 g of Na2HPO4•2H2O or 2.68 g of Na2HPO4•7H2O (Prod. # S745)
1.38 g of NaH2PO4•H2O (Prod # S515)

It has been reported that low concentrations of GAs (0.01-5.0 mg/L) have been shown to promote shoot development in tobacco callus, while higher concentrations (5.0 mg/L to 10 mg/L) inhibit shoot production. 4
PhytoTechnology Laboratories® also carries Gibberellic Acid Solution at 13 mg/mL (Prod. No. G362), Gibberellic Acid Solution at 1 mg/mL (Prod. No. G198), and Gibberellins A4+A7 (Prod. No. G358).

Please Note: Gibberellins can be co-autoclaved with media components; however, some loss of activity may occur. While PhytoTechnology Laboratories™ tests each lot of this product with two or more plant cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used

References
1. Merck 13, 4430
2. Harada H. and J. P. Nitsch (1959) Flower Induction in Japanese Chrysanthemums with Gibberellic Acid. Science , New Series 129;335, pp. 777-778.
3. Canadian Food Inspection Agency (2011) Canadian Methods and Procedures for Testing Seed. www.aosaseed.com/docs/Canadian_M&P_2011.pdf
4. Murashige, T (1961) Suppression of Shoot Formation in Cultured Tobacco Cells by Gibberellic Acid. Science, New Series, 134:3474, pp. 280.