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PhytoTechnology Laboratories

CTAB
Product ID: H276

Introduction

Description: CTAB is a cationic surfactant that has been used to complex and precipitate high MW plant DNA. Its activity is neutralized by anionic surfactants (e.g. SDS).CTAB has bactericidal activity against both gram-positive and gram-negative organisms. It can be used with thePhytoSelect Basal Medium (P6800) for the selection of Burkholderia glumae, Acidovorax avenae, andPectobacterium carotovorum. See product information sheet for P6800 for details.Synonyms: CTAB; HTAB; Centimide; Cetrimide; Cetrimonium bromide; Cetyltrimethylammonium Bromide; Palmityltrimethylammonium bromide; Hexadecyltrimethylammonium Bromide

Solubility Water
Use With P6800 - PhytoSelect Basal Medium
Physical Form Solid
Solutions Available H3818- 100 mg/mL
Storage Temp. Room Temperature
UPC/SKU H276
CAS NUMBER 57-09-0
Formula Weight 364.45
Formula C19H42NBr
Synonyms CTAB; HTAB; Centimide; Cetrimide; Cetrimonium bromide; Cetyltrimethylammonium Bromide; Palmityltrimethylammonium bromide; Hexadecyltrimethylammonium Bromide
Storage Temp. Room Temperature
Tariff Code 2923.90.0000
Risk Info (R) 36/37/38-23/34/35-34
Safety Info (S) 22-26-36/37/39
No information available

H276 HEXADECYLTRIMETHYLAMMONIUM BROMIDE

Synonyms: CTAB, HTAB, Centimide, Cetrimide, Cetrimonium bromide
CAS: 59-09-0
Formula: C19H42NBr
Molecular Wt: 364.45
Properties
Form: Powder
Appearance: White to Off-White Powder
Application: Molecular Biology
Solubility: Soluble in Water
Typical Working Concentration: 10-20 g/L
Storage Temp: Room Temperature

Application Notes
CTAB is a cationic detergent that is often used to complex and precipitate high molecular weight DNA when the NaCl concentration drops from 0.7M to 0.35M (Murray and Thompson 1980). Its activity is neutralized by anionic detergents (such as SDS). It also exhibits bactericidal activity against both gram positive and negative organisms.

References
Merck 13, 2034.

M.G.Murray and W.F.Thompson (1980). Rapid isolation of high molecular weight plant DNA. Nuc. Acids Res. Vol 8(19) pg. 4321-4325.