Introduction
Description: Hanahan's Broth (SOB Medium) was developed by Douglas Hanahan (1983) for the cultivation of competentEscherichia coli cells prior to transformation.SOB Medium is a nutrient-rich, bacterial growth medium that is used for microbiological culture, generally of E. coli.
When 20 mM (0.36%) D-glucose is added to this nutritionally rich medium it is referred to as SOC (Super Optimal broth with Catabolite repression) Medium. It is used in the final stage of transformation; this allows the E. coli cells to mend the perforations and replicate.Synonyms: Super Optimal Broth; SOB
| Solubility |
Water |
| Physical Form |
Solid |
| Solutions Available |
S657 - SOC Medium, 1X (H289 + 20 mM glucose) |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
28.09 |
| UPC / SKU |
H289 |
| Synonyms |
Super Optimal Broth; SOB |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3821.00.0000 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| M150 |
Magnesium Sulfate, Anhydrous |
2400 |
| P704 |
Potassium Chloride |
186 |
| S624 |
Sodium Chloride |
500 |
| T832 |
Tryptone |
20,000 |
| Y892 |
Yeast Extract |
5000 |
H289 Hanahan’s Broth (SOB Medium)
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Off-White to Light Tan |
| Application: |
Molecular Biology |
| Solubility: |
Water |
| Typical Working Concentration: |
28.09 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Storage of stock solutions is not recommended as precipitates may form. |
| Other Notes: |
Approximate pH of 7.0 |
| Formula (mg/L) |
|---|
| Magnesium Sulfate, Anhydrous |
2400 |
| Potassium Chloride |
186 |
| Sodium Chloride |
500 |
| Tryptone |
20,000 |
| Yeast Extract |
5000 |
Application Notes
Also known as SOB Medium. It was developed by Hanahan (1983) for the cultivation of competent E. coli cells prior to transformation. When 20 mM (0.36%) D-glucose is added to this nutritionally rich medium it is used in the final stage of transformation; this allows the E. coli cells to mend the performations and replicate
Adding D-glucose to SOB Medium produces SOC Medium (Prod. No. S657).
References
Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 155, 557–508.
