Introduction
Description: Contains the macro- and micronutrients as described by Heller (1953).
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
1.64 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
H393 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A127 |
Aluminum Chloride, Heptahydrate |
0.0543 |
| B210 |
Boric Acid |
1.0 |
| C266 |
Calcium Chloride, Anhydrous |
56.7 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.03 |
| F383 |
Ferric Chloride |
1.0 |
| M150 |
Magnesium Sulfate, Anhydrous |
122.1 |
| M250 |
Manganese Sulfate |
0.0758 |
| N478 |
Nickel Chloride Hexahydrate |
0.03 |
| P704 |
Potassium Chloride |
750 |
| P840 |
Potassium Iodide |
0.01 |
| S802 |
Sodium Nitrate |
600 |
| S515 |
Sodium Phosphate, Monobasic |
108.75 |
| Z101 |
Zinc Sulfate, Heptahydrate |
1.0 |
H393 Heller Basal Salt Mixture
| Properties |
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
1.64g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients as described by Heller (1953). |
| Formula (mg/L) |
| Aluminum Chloride·6H2O |
0.0543 |
| Boric Acid |
1.0 |
| Calcium Chloride, Anhydrous |
56.7 |
| Cupric Sulfate·5H2O |
0.03 |
| Ferric Chloride |
1.0 |
| Magnesium Sulfate, Anhydrous |
122.1 |
| Manganese Sulfate·H2O |
0.0758 |
| Molybdenum Trioxide |
0.03 |
| Potassium Chloride |
750 |
| Potassium Iodide |
0.01 |
| Sodium Nitrate |
600 |
| Sodium Phosphate Monobasic |
108.75 |
| Zinc Sulfate·7H2O |
1.0 |
Application Notes
Plant tissues: Cocos nucifera (Nurita-Toruan, 1978); Digitalis purpurea (Rucker et al, 1976); Elaeis guineensis (Buffard-Morel, 1968); Laeliocattleya (Churchill et al, 1971); Magnolia soulangeana (Le PageDegivry, 1970); Paphiopedilum spp. (Stewart & Button, 1975).
References
Buffard-Morel J (1968) Effets du glucose, du lévulose, du maltose et du saccharose sur le développement des embryons de palmier à huile (Elaeis guineensis Jacq. Var. Dura Bec)
en culture in vitro. Comptes Rendus de l'Académie des Sciences.Série D: Sciences Naturelles (2), p. 185-188. ISSN 0249-6305 Churchill ME, Arditti J & Ball EA (1971) Clonal propagation of orchids from leaf tips.
Am. Orchid Soc. Bull. 40: 109-113. Heller R. (1953) Researches on the mineral nutrition of plant tissues.
Ann. Sci. Nat. Bot. Biol. Veg.,11th Ser. 14: 1–223. Le Page-Degivry MT (1970) [Seed dormancy associated with embryo immaturity: Contribution for the study of Magnolia soulangeana Soul. Bod. and Magnolia grandiflora L. by means of in vitro culture]. Planta. 90(3):267-71. doi: 10.1007/BF00387178.
Nurita-Toruan (1978) Pertumbuhan dan perkembangan embrio kelapa (Cocos nucifera L.) dalam kultur aseptik. [Indonesian] Menara Perkebunan 46(5): 213-217.