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Heller Basal Salt Mixture
Product ID: H393


Description: Contains the macro- and micronutrients as described by Heller (1953).

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 1.64
Other Notes Plant Tissue Culture Tested
UPC / SKU H393
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A127 Aluminum Chloride, Heptahydrate 0.0543
B210 Boric Acid 1.0
C266 Calcium Chloride, Anhydrous 56.7
C375 Cupric Sulfate, Pentahydrate 0.03
F383 Ferric Chloride 1.0
M150 Magnesium Sulfate, Anhydrous 122.1
M250 Manganese Sulfate 0.0758
N478 Nickel Chloride Hexahydrate 0.03
P704 Potassium Chloride 750
P840 Potassium Iodide 0.01
S802 Sodium Nitrate 600
S515 Sodium Phosphate, Monobasic 108.75
Z101 Zinc Sulfate, Heptahydrate 1.0

H393 Heller Basal Salt Mixture

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 1.64g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Heller (1953).
Formula (mg/L)
Aluminum Chloride·6H2O 0.0543
Boric Acid  1.0
Calcium Chloride, Anhydrous 56.7
Cupric Sulfate·5H2O 0.03
Ferric Chloride  1.0
Magnesium Sulfate, Anhydrous  122.1
Manganese Sulfate·H2O 0.0758
Molybdenum Trioxide  0.03
Potassium Chloride  750
Potassium Iodide  0.01
Sodium Nitrate 600
Sodium Phosphate Monobasic  108.75
Zinc Sulfate·7H2O  1.0

Application Notes
Plant tissues: Cocos nucifera (Nurita-Toruan, 1978); Digitalis purpurea (Rucker et al, 1976); Elaeis guineensis (Buffard-Morel, 1968); Laeliocattleya (Churchill et al, 1971); Magnolia soulangeana (Le PageDegivry, 1970); Paphiopedilum spp. (Stewart & Button, 1975).

Buffard-Morel J (1968) Effets du glucose, du lévulose, du maltose et du saccharose sur le développement des embryons de palmier à huile (Elaeis guineensis Jacq. Var. Dura Bec)
en culture in vitro. Comptes Rendus de l'Académie des Sciences.Série D: Sciences Naturelles (2), p. 185-188. ISSN 0249-6305 Churchill ME, Arditti J & Ball EA (1971) Clonal propagation of orchids from leaf tips.
Am. Orchid Soc. Bull. 40: 109-113. Heller R. (1953) Researches on the mineral nutrition of plant tissues.
Ann. Sci. Nat. Bot. Biol. Veg.,11th Ser. 14: 1–223. Le Page-Degivry MT (1970) [Seed dormancy associated with embryo immaturity: Contribution for the study of Magnolia soulangeana Soul. Bod. and Magnolia grandiflora L. by means of in vitro culture]. Planta. 90(3):267-71. doi: 10.1007/BF00387178.
Nurita-Toruan (1978) Pertumbuhan dan perkembangan embrio kelapa (Cocos nucifera L.) dalam kultur aseptik. [Indonesian] Menara Perkebunan 46(5): 213-217.