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PhytoTechnology Laboratories

Hosta Initiation/Multiplication Medium II
Product ID: H3959


Description: A modification of the macro- and micronutrients of Murashige and Skoog Basal Medium with vitamins, PGRs, Sucrose and Agar.

Solubility Partially in Water
Physical Form Solid
Storage Temp. 2-6 C
Grams of powder to prepare 1 Liter 43.4
Other Notes Plant Tissue Culture Tested
UPC / SKU H3959
Storage Temp. 2-6 C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 300
S515 Sodium Phosphate, Monobasic 170
Z101 Zinc Sulfate, Heptahydrate 8.6
A545 Adenine Hemisulfate 160
A111 Agar 8000
B800 6-Benzylaminopurine (6-BA) 2.0
G503 Glycine 2.0
I703 myo-Inositol 100
N600 a-Naphthaleneacetic Acid 0.02
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.4
M561 Murashige & Skoog Modified Basal Salt Mixture

H3959 Hosta Inititation/Multiplication Medium II

Form: Powder
Appearance: White to Off-White
Application: Plant Tissue Culture
Solubility: Partially Soluble in Water
Typical Working Concentration: 43.40 g/L
Storage Temp: 2-6C
Other Notes: Plant Tissue Culture Tested
Formula (mg/L)
6-Benzylaminopurine 2
Adenine Hemisulfate 160
Agar 8000
Ammonium Nitrate 1650
a-Napththaleneacetic Acid 0.02
Boric Acid 6.2
Calcium Chloride Anhydrous 332.2
Casein Hydrolysate 500
Cobalt Chloride Hexahydrate 0.025
Cupric Sulfate Pentahydrate 0.025
Ferrous Sulfate Heptahydrate 27.8
Glycine 2
Magnesium Sulfate Anhydrous 180.7
Magnesium Sulfate Monohydrate 16.9
Molybdic Acid, Sodium Salt 0.25
Myo-Inositol 100
Na2 EDTA Dihydrate 37.26
Potassium Iodide 0.83
Potassium Iodide 1900
Potassium Phosphate Monobasic 300
Sodium Phosphate Monobasic 170
Sucrose 30000
Thiamine HCl 0.4
Zinc Sulfate, Heptahydrate 8.6

Application Notes
Same formulation as medium H436, except this formulation has a reduced amount of NAA.