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PhytoTechnology Laboratories

Hosta Initiation/Multiplication Medium
Product ID: H435


Description: Stage I/II medium. Modified Murashige & Skoog with (mg/L): 2.0 6-BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, & 0.5 NAA.

Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and the vitamins described by Linsmaier & Skoog (1965). Also contains sucrose and agar.

Solubility Water (Partially)
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 43.4
Other Notes Plant Tissue Culture Tested
UPC / SKU H435
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 300
M651 Sodium Molybdate (VI), Dihydrate 0.25
S515 Sodium Phosphate, Monobasic 170
Z101 Zinc Sulfate, Heptahydrate 8.6
A545 Adenine Hemisulfate 160
A111 Agar 8000
B800 6-Benzylaminopurine (6-BA) 2.0
C184 Casein, Enzymatic Hydrolysate 500
G503 Glycine 2.0
I703 myo-Inositol 100
N600 a-Naphthaleneacetic Acid 0.5
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.4

H435 Hosta Initiation/ Multiplication Medium Stage I/II Medium

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water
Typical Working Concentration: 43.39 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains agar and sucrose. Plant Tissue Culture Tested.
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
 Calcium Chloride, Anhydrous 332.2
 Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Disodium EDTA .2H2O 37.26
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
 Molybdic Acid (Sodium Salt). 2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 300
Sodium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6
 Adenine Hemisulfate 160
Agar 8000
6-Benzylaminopurine (BA) 2.0
 Casein, Enzymatic Hydrolysate 500
 Glycine (Free Base)  2 .0
myo-Inositol  100
a-Naphthaleneacetic Acid  0.5
 Sucrose 30,000
Thiamine.HCl  0.4

Application Notes
Plant species: Hosta. Excellent stage I and II medium for Hosta cultures

Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.