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Hosta Multiplication Medium
Product ID: H436


Description: Stage II medium. Modified M & S with (mg/L): 0.1 6-BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, & 0.5 NAA.

Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and the vitamins described by Linsmaier & Skoog (1965). Also contains sucrose andagar.

Solubility Partially Soluble in Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 43.39
Other Notes Plant Tissue Culture Tested
UPC / SKU H436
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 300
M651 Sodium Molybdate (VI), Dihydrate 0.25
S515 Sodium Phosphate, Monobasic 170
Z101 Zinc Sulfate, Heptahydrate 8.6
A545 Adenine Hemisulfate 160
A111 Agar 8000
B800 6-Benzylaminopurine (6-BA) 0.1
C184 Casein, Enzymatic Hydrolysate 500
G503 Glycine 2.0
I703 myo-Inositol 100
N600 a-Naphthaleneacetic Acid 0.5
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.4

H436 Hosta Multiplication Medium Stage II Medium

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Partially Soluble in Cold Water. Soluble in Hot Water
Typical Working Concentration: 43.39 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains agar and sucrose. Plant Tissue Culture Tested
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride•6H2O 0.025
Cupric Sulfate•5H2O 0.025
Disodium EDTA •2H2O 37.26
Ferrous Sulfate•7H2O 27.8
 Magnesium Sulfate, Anhydrous  180.7
 Manganese Sulfate.H2O 16.9
 Molybdic Acid (Sodium Salt). 2H2O  0.25
 Potassium Iodide 0.83
 Potassium Nitrate  1900
 Potassium Phosphate, Monobasic  300
 S odium Phosphate, Monobasic  170
 Zinc Sulfate.7H2O 8.6
 Adenine Hemisulfate 160
Agar  8000
 6-Benzylaminopurine (BA) 0.1
 Casein, Enzymatic Hydrolysate 500
 Glycine (Free Base) 2
 myo-Inositol 100
 a-Naphthaleneacetic Acid  0.5
 Sucrose  30,000
 Thiamine.HCl 0.4

Application Notes
Plant species: Hosta

Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.