ATZ Labs - a subsidiary of Life Technologies (India) Pvt. Ltd.
Life Technologies India
PhytoTechnology Laboratories

Ichihashi New Phalaenopsis (NP) Medium
Product ID: I365


Description: Contains the components as described Ichihashi (1992); modified to contain 82.0 mg/L Ammonium Nitrate.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 25.35
Other Notes Plant Tissue Culture Tested
UPC / SKU I365
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 82
A305 Ammonium Sulfate 303.9
B210 Boric Acid 3.1
C180 Calcium Nitrate 637.6
C350 Cobalt Chloride Hexahydrate 0.0125
C375 Cupric Sulfate, Pentahydrate 0.0125
E410 EDTA, Disodium Salt 37.3
F263 Ferrous Sulfate, Heptahydrate 27.8
M542 Magnesium Nitrate 256.4
M250 Manganese Sulfate 11.2
P840 Potassium Iodide 0.415
P100 Potassium Nitrate 424
P846 Potassium Phosphate, Monobasic, Anhydrous 462.7
M651 Sodium Molybdate (VI), Dihydrate 0.125
Z101 Zinc Sulfate, Heptahydrate 4.3
G434 Gellan Gum (CultureGelT) 3000
G503 Glycine 2
I703 myo-Inositol 100
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
S391 D-Sucrose 20,000
T390 Thiamine HCl 0.1

I365 Ichihashi New Phalaenopsis (NP) Medium

Form: Powder
Appearance: White to Cream
Application: Plant Tissue Culture
Solubility: Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water.
Typical Working Concentration: 25.35 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: N/A
Other Notes: Contains the components as described Ichihashi (1992); modified to contain 82.0 mg/L Ammonium Nitrate. Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Formula (mg/L)
Ammonium Nitrate  82
 Ammonium Sulfate  303.9
 Boric Acid 3.1
 Calcium Nitrate 637.6
 Cobalt Chloride.6H2O 0.0125
 Cupric Sulfate.5H2O 0.0125
 Na2 EDTA.2H2O 37.3
Ferrous Sulfate.7H2O  27.8
 Magnesium Nitrate 256.4
 Manganese Sulfate.H2O 11.2
 Molybdic Acid (Sodium Salt) .2H2O 0.125
 Potassium Iodide  0.415
 Potassium Nitrate  424
 Potassium Phosphate, Monobasic 462.7
 Zinc Sulfate.7H2O 4.3
 Gellan Gum, CultureGel - Biotech 3000
 Glycine (Free Base)  2
 myo-Inositol  100
Nicotinic Acid (Free Acid)  0.5
 Pyridoxine.HCl 0.5
 Sucrose 20,000
 Thiamine.HCl 0.1

Application Notes
Plant Tissue Culture Tested
Plant species: Phalaenopsis spp.
This medium was originally developed for the stem propagation of Phalaenopsis.

Ichihashi, S. 1992. Abstr. of IPPS Toyohashi 98.