Introduction
Description: Used as a tissue stain, pH indicator and viability stain.
As a pH indicator, it is blue at pH 11.4 and yellow at 13.0.As a viability stain, it will stain dead cells blue while living cells remain unstained.
Description:
A biological stain used to test light-colored seeds for mechanical damage.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| UPC / SKU |
I4068 |
| CAS NUMBER |
860-22-0 |
| Formula Weight |
466.35 |
| Formula |
C16H8N2Na2O8S2 |
| Synonyms |
Acid Blue 74; Indigo-5,5'-disulfonic acid disodium salt; Indigocarmine; C.I. 73015 |
| Storage Temp. |
Room Temperature |
| Tariff Code |
3204.19.5000 |
| Risk Info (R) |
22-36/37/38 |
| Safety Info (S) |
22-24/25-36/37/39-27-26 |
No information available
I4068 Indigo Carmine
| Synonyms: |
5,5'-Indigodisulfonic Acid Sodium Salt, Indigotine, Acid Blue 74, CI#: 73015 |
| CAS: |
860-22-0 |
| Formula: |
C16H8N2Na2O8S2 |
| Mol. Weight: |
466.35 |
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Dark Violet |
| Application: |
Biological Stain, Seed Testing |
| Solubility: |
Soluble in Water, Ethanol |
| Typical Working Concentration: |
Varies with applications, should be determined by end user. |
| Storage Temp: |
Room Temperature |
Application Notes
Used to stain seeds to detect dead tissues. Stain will penetrate dead cells, but will not stain living cells. This can aid in determining seed viability (Gosling 2003). Indigo Carmine can also be used as a pH indicator: It is blue at pH 11.4 and yellow at 13.0.
References
Gadd, I. & A. Kjaer (1940) On the applicability of the selenite and indigo-carmine methods to the testing of frostand Fusarium- damaged cereal seed-grain. Proceedings of the International Seed Testing Association Vol. 12 No. 2 pp. 140-149. Gosling PG (2003) Viability testing. In: Smith RD, Dickie JB, Linington SL, Pritchard HW, Probert RJ (eds) Seed conservation turning science into practice. Royal Botanic Gardens, Kew, pp 445–481.
