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Indigo Carmine
Product ID: I4068


Description: Used as a tissue stain, pH indicator and viability stain.
As a pH indicator, it is blue at pH 11.4 and yellow at 13.0.As a viability stain, it will stain dead cells blue while living cells remain unstained.

A biological stain used to test light-colored seeds for mechanical damage.

Solubility Water
Physical Form Solid
Storage Temp. Room Temperature
UPC / SKU I4068
CAS NUMBER 860-22-0
Formula Weight 466.35
Formula C16H8N2Na2O8S2
Synonyms Acid Blue 74; Indigo-5,5'-disulfonic acid disodium salt; Indigocarmine; C.I. 73015
Storage Temp. Room Temperature
Tariff Code 3204.19.5000
Risk Info (R) 22-36/37/38
Safety Info (S) 22-24/25-36/37/39-27-26
No information available

I4068 Indigo Carmine

Synonyms: 5,5'-Indigodisulfonic Acid Sodium Salt, Indigotine, Acid Blue 74, CI#: 73015
CAS: 860-22-0
Formula: C16H8N2Na2O8S2
Mol. Weight: 466.35
Form: Powder
Appearance: Dark Violet
Application: Biological Stain, Seed Testing
Solubility: Soluble in Water, Ethanol
Typical Working Concentration: Varies with applications, should be determined by end user.
Storage Temp: Room Temperature

Application Notes
Used to stain seeds to detect dead tissues. Stain will penetrate dead cells, but will not stain living cells. This can aid in determining seed viability (Gosling 2003). Indigo Carmine can also be used as a pH indicator: It is blue at pH 11.4 and yellow at 13.0.

Gadd, I. & A. Kjaer (1940) On the applicability of the selenite and indigo-carmine methods to the testing of frostand Fusarium- damaged cereal seed-grain. Proceedings of the International Seed Testing Association Vol. 12 No. 2 pp. 140-149. Gosling PG (2003) Viability testing. In: Smith RD, Dickie JB, Linington SL, Pritchard HW, Probert RJ (eds) Seed conservation turning science into practice. Royal Botanic Gardens, Kew, pp 445–481.