Introduction
Description: Morel modification with the macro- and micronutrients as described by Knudson (1946).
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
22 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
K400 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
500 |
| A305 |
Ammonium Sulfate |
500 |
| C180 |
Calcium Nitrate |
347.2 |
| F263 |
Ferrous Sulfate, Heptahydrate |
25 |
| M150 |
Magnesium Sulfate, Anhydrous |
122.125 |
| M250 |
Manganese Sulfate |
5.682 |
| P704 |
Potassium Chloride |
250 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
250 |
| S391 |
D-Sucrose |
20,000 |
K400
Knudson C Orchid Medium
Morel Modification
| Properties |
| Form: |
Powder |
| Appearance: |
White to Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Soluble in
Water |
| Typical Working Concentration: |
22.0
g/L |
| Storage Temp: |
2-6° C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form. |
| Other Notes: |
Morel modification with the macro
-
and micronutrients and sucrose as
described by K
nudson (1946).
pH = 4.0
-
5.0
.
Plant Tissue Culture Test |
| Formula (mg/L) |
| Ammonium Nitrate |
500 |
| Ammonium Sulfate |
500 |
| Calcium Nitrate |
347.2 |
| Ferrous Sulfate.7H2O |
25 |
| Magnesium Sulfate, Anhydrous |
122.13 |
| Manganese Sulfate.H2O |
5.68 |
| Potassium Chloride |
250 |
| Potassium Phosphate, Monobasic |
250 |
| Sucrose |
20,000 |
Application Notes
Plant species:
Cymbidium
This medium was originally developed for the germination of Cymbidium orchid s
References
Knudson, L. 1946.
A new nutrient solution for germination of
orchid
seed.
A
mer. Orchid Soc.
Bull. 15:
214
-217.
Morel, GM. 1965. Clonal propagation
of orchids by meristem culture.
Cymb. Soc. News
20
:3
-11