Introduction
Description: Kao & Michayluk vitamin solution (100x).
Use at a concentration of 10 mL/L of medium to achieve the proper final concentration.
| Miscibility |
Water |
| Physical Form |
Liquid |
| Solutions Available |
100x |
| Sterility |
Sterile Filtered |
| Storage Temp. |
-20 °C |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
K421 |
| Storage Temp. |
-20 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A103 |
?-Aminobenzoic Acid |
2 |
| A106 |
L-Ascorbic Acid |
200 |
| B140 |
D-Biotin |
1 |
| C186 |
Calcium Pantothenate |
100 |
| C232 |
Choline Chloride |
100 |
| C242 |
Cyanocobalamin, Vitamin B12 |
2 |
| F430 |
Folic Acid |
40 |
| I703 |
myo-Inositol |
10,000 |
| N609 |
Niacinamide |
100 |
| P866 |
Pyridoxine HCl |
100 |
| R779 |
Riboflavin, Vitamin B2 |
20 |
| T390 |
Thiamine HCl |
100 |
K421
Kao & Michayluk Vitamin Solution (100x)
| Properties |
|---|
| Form: |
Liquid |
| Appearance: |
Clear, Colorless Solution |
| Application: |
Plant Tissue Culture |
| Solubility: |
Miscible with Water |
| Typical Working Concentration: |
10.0 mL/L of Medium |
| Storage Temp: |
--20-0 °C |
| Other Notes: |
Contains the vitamins as described by Kao and Michayluk (1975). Sterile
Filtered. |
| Formula (mg/L) |
|---|
| p-Aminobenzoic Acid |
2 |
| myo-Inositol |
10,000 |
| L-Ascorbic Acid |
200 |
| Niacinamide |
100 |
| D-Biotin |
1 |
| Pyridoxine.HCl |
100 |
| D-Calcium Pantothenate |
100 |
| Retinol; Vitamin A |
1 |
| Choline Chloride |
100 |
| Riboflavin |
20 |
| Cyancobalamin; Vit B12 |
2 |
| Thiamine.HCl |
100 |
Application Notes
Plant species: Vicia hajastana (Kao & Michayluk, 1975); Vicia faba (Binding & Nehls, 1978);
Daucus carota (Grzebelus et al, 2012).
References
Binding, H. and Nehls, R. (1978) Regeneration of Isolated Protoplasts of Vicia faba L. Z. Pflanzenpbysiol. 88, 327-332.
Grzebelus E, M Szklarczyk & R Baranski (2012) An improved protocol for plant regeneration from leafand hypocotyl-derived protoplasts of carrot. Plant Cell Tiss Organ Cult 109:101-109.
Kao, KN and MR Michayluk (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.
