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Kao & Michayluk Modified Basal Salt Medium
Product ID: K427


Description: Contains the macro- & micronutrients, vitamins, & organic acids as described by Kao & Michayluk.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 3.9
Other Notes Plant Tissue Culture Tested
UPC / SKU K427
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 600
B210 Boric Acid 3.0
C266 Calcium Chloride, Anhydrous 453
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.85
M150 Magnesium Sulfate, Anhydrous 146.55
M250 Manganese Sulfate 10
P704 Potassium Chloride 300
P840 Potassium Iodide 0.75
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 2.0
A103 ?-Aminobenzoic Acid 0.02
A106 L-Ascorbic Acid 2.0
B140 D-Biotin 0.01
C186 Calcium Pantothenate 1.0
V883 Cholecalciferol, Vitamin D3 0.01
C232 Choline Chloride 1.0
C277 Citric Acid, Anyhdrous 40
C242 Cyanocobalamin, Vitamin B12 0.02
F430 Folic Acid 0.4
F356 Fumaric Acid 40
I703 myo-Inositol 100
M5536 DL-Malic Acid 40
N609 Niacinamide 1.0
P866 Pyridoxine HCl 1.0
P684 Pyruvic Acid, Potassium Salt 20
R779 Riboflavin, Vitamin B2 0.2
T390 Thiamine HCl 1.0


Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 3.9g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins and organic supplements as described by Kao and Michayluk (1975).
Formula (mg/L)
Ammonium Nitrate 600
Boric Acid  3
Calcium Chloride, Anhydrous 453
Cobalt Chloride·6H2O  0.025
Cupric Sulfate·5H2O  0.025
Na2 EDTA·2H2O  37.26
Ferrous Sulfate·7H2O  27.85
Magnesium Sulfate, Anhydrous  146.55
Manganese Sulfate·H2O 10
Molybdic Acid (Sodium Salt)·2H2O 0.25
Potassium Chloride 300
Potassium Iodide 0.75
Potassium Nitrate 1900
Potassium Phosphate, Monobasic  170
Zinc Sulfate·7H2O  2
p-Aminobenzoic Acid 0.02
L-Ascorbic Acid  2
D-Biotin  0.01
D-Calcium Pantothenate 1
Choline Chloride  1
Citric Acid (Free Acid) Anhydrous  40
Cyanocobalamin (Vitamin B12) 0.02
Folic Acid  0.4
Fumaric Acid  40
DL-Malic Acid 40
myo-Inositol  100
Niacinamide  1
Pyridoxine·HCl 1
Pyruvic Acid 20
Riboflavin  0.2
Thiamine·HCl  1
Vitamin A  0.01
Vitamin D3 0.01

Application Notes
Plant species: Vicia hajastana (Kao & Michayluk, 1975); Vicia faba (Binding & Nehls, 1978); Daucus carota (Grzebelus et al, 2012).

This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/ml) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. Cell growth and division could also be enhanced by raising the concentrations of calcium chloride from 1 to 5 mM.

Binding, H. and Nehls, R. (1978) Regeneration of Isolated Protoplasts of Vicia faba L. Z. Pflanzenpbysiol. 88, 327-332. Grzebelus E, M Szklarczyk & R Baranski (2012) An improved protocol for plant regeneration from leafand hypocotyl-derived protoplasts of carrot.
Plant Cell Tiss Organ Cult 109:101-109. Kao, KN and MR Michayluk (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.