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Lloyd & McCown Woody Plant Basal Medium with Vitamins
Product ID: L449

Introduction

Description: Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981).
This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium Nitrate has been eliminated from this medium and replaced with Potassium Sulfate.

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 2.41
Other Notes Plant Tissue Culture Tested
UPC / SKU L449
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 400
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 72.5
C180 Calcium Nitrate 386
C375 Cupric Sulfate, Pentahydrate 0.25
E410 EDTA, Disodium Salt 37.3
F263 Ferrous Sulfate, Heptahydrate 27.85
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 22.3
P846 Potassium Phosphate, Monobasic, Anhydrous 170
P854 Potassium Sulfate, Anhydrous 990
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
G503 Glycine 2.0
I703 myo-Inositol 100
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
T390 Thiamine HCl 1.0

L449 Lloyd & McCown Woody Plant Basal Medium

Synonym: WPM

Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 2.41g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate  400
Boric Acid 6.2
Calcium Chloride, Anhydrous  72.5
Calcium Nitrate 386
Cupric Sulfate.5H2O  0.25
Na2 EDTA.2H2O  37.3
Ferrous Sulfate.7H2O 27.85
Magnesium Sulfate, Anhydrous  180.7
Manganese Sulfate.H2O  22.3
Molybdic Acid (Sodium Salt) .2H2O  0.25
Potassium Phosphate, Monobasic  170
Potassium Sulfate  990
Zinc Sulfate.7H2O 8.6
Glycine (Free Base)  2
myo-Inositol 100
Nicotinic Acid (Free Acid) 0.5
Pyridoxine.HCl 0.5
Thiamine.HCl  1

Application Notes
Plant Tissue Culture Tested
Plant species: Kalmia latifolia (Mountain Laurel), Rhododendron spp. This medium was originally developed for the culture of shoot tips of Mt. Laurel. The medium has since become a standard for the culture of many woody plant species. Potassium nitrate has been eliminated from this medium and replaced with potassium sulfate. The levels of ammonium nitrate are ¼x compared to MS. Nitrogen is also provided by calcium nitrate.

References
Lloyd, G and BH McCown. 1981. Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture. Proc. Int. Plant Prop. Soc. 30:421-427.