Introduction
Description: Contains the macro-& micronutrients, & vitamins as described by L & S (1964). With 30 g/L sucrose & 7 g/L agar. pH adjusted and buffered.
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR's, gelling agent, or other supplements. The addition of MES will help to stabilize pH fluctuation during media preparation and culture.
| Solubility |
Partially Soluble in Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
42.53 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
L452 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
1650 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
332.2 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.26 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
| M250 |
Manganese Sulfate |
16.9 |
| P704 |
Potassium Chloride |
100 |
| P672 |
Potassium Hydroxide |
0.83 |
| P840 |
Potassium Iodide |
1900 |
| P100 |
Potassium Nitrate |
170 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z897 |
Zinc Nitrate, Hexahydrate |
8.6 |
| A111 |
Agar |
7000 |
| I703 |
myo-Inositol |
100 |
| M825 |
MES, Free Acid |
1000 |
| S391 |
D-Sucrose |
30,000 |
| T390 |
Thiamine HCl |
0.4 |
L452 Linsmaier & Skoog Modified Basal Medium w/ 30 g/L Sucrose & 7 g/L Agar pH Adjusted and Buffered
Synonym: Murashige & Skoog (MS) Medium with Minimal Organics (MSMO)
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow |
| Application: |
Plant Tissue Culture |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
42.53 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Linsmaier and Skoog (1965) |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
1650 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
332.2 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2 EDTA.2H2O |
37.26 |
| Ferrous Sulfate.7H2O |
27.8 |
| Magnesium Sulfate |
180.7 |
| Manganese Sulfate.H2O |
16.9 |
| Molybdic Acid (Sodium Salt) .2H2O |
0.25 |
| Potassium Hydroxide |
100 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
1900 |
| Potassium Phosphate, Monobasic |
170 |
| Zinc Sulfate.7H2O |
8.6 |
| Agar |
7000 |
| MES (Free Acid) |
1000 |
| myo-Inositol |
100 |
| Sucrose |
30,000 |
| Thiamine.HCl |
0.4 |
Application Notes
Plant Tissue Culture Tested
This medium is the standard Murashige & Skoog (MS) basal salts supplemented with Linsmaier and Skoog vitamins. The medium is pH adjusted and buffered with MES. Under most circumstances, adjusting the pH is not necessary; simply add water and any desired PGR’s or other supplements.
The addition of MES will help to stabilize pH fluctuations during media preparation and culture
References
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18:100-127.
