Introduction
Description: Contains the nutrients as described by Bertani (1951), and Luria & Burrows (1955). Formulation modified by Miller (1972). This medium is used to grow Escherichia coli for molecular biology applications. This is a rich medium that promotes rapid growth of pure cultures of recombinant strains. This is the most widely-used formulation of LB Broth, Stambrook (1989). Also known as Lysogeny Broth or Luria-Bertani Broth.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
25 |
| UPC / SKU |
L475 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3821.00.0000 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| S624 |
Sodium Chloride |
10,000 |
| T832 |
Tryptone |
10,000 |
| Y892 |
Yeast Extract |
5000 |
L475 LB Broth Luria-Bertani Broth Miller Modification
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Yellow to Cream Powder |
| Application: |
Microbiology |
| Solubility: |
Soluble in Water |
| Typical Working Concentration: |
25.00 g/L |
| Storage Temp: |
2 – 6° C |
| Other Notes: |
pH 6.5 – 7.5 |
