Life Technologies India
PhytoTechnology Laboratories

Musa (Banana) Multiplication Medium
Product ID: M462


Description: Contains the macro- and micronutrients as described by Murashige and Skoog (1962).
IITA formulation as described by Vuylsteke (1989) (International Institute for Tropical Agriculture).

Solubility Water (Partially)
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 36.36
Other Notes Plant Tissue Culture Tested
UPC / SKU M462
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.25
F263 Ferrous Sulfate, Heptahydrate 27.85
M150 Magnesium Sulfate, Anhydrous 180.74
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6
A106 L-Ascorbic Acid 20
B800 6-Benzylaminopurine (6-BA) 4.5
G434 Gellan Gum (CultureGelT) 2000
G503 Glycine 2
I885 Indole-3-acetic Acid 0.175
N765 Nicotinic Acid 0.5
P866 Pyridoxine HCl 0.5
S391 D-Sucrose 30,000
T390 Thiamine HCl 0.4

M462 Musa (Banana) Multiplication Medium IITA Formulation*

Form: Powder
Appearance: White to Off-White
Application: Plant Tissue Culture
Solubility: Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water.
Typical Working Concentration: 36.36 g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Formulation as described by Vuylsteke (1989).
pH = 3.75 - 4.75
*International Institute for Tropical Agriculture
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O  0.025
Cupric Sulfate.5H2O  0.025
Na2EDTA.2H2O  37.26
Ferrous Sulfate  27.85
Magnesium Sulfate, Anhydrous  180.74
Manganese Sulfate.H2O  16.9
Molybdic Acid (Sodium Salt).2H2O  0.25
Potassium Iodide  0.83
Potassium Nitrate  1900
Potassium Phosphate Monobasic  170
Zinc Sulfate.7H2O  8.6
L-Ascorbic Acid  20
6-Benzylaminopurine (BA)  4.5
Gellan Gum - Biotech Grade  2000
Glycine  2
Indole-3-acetic Acid  0.175
Nicotinic Acid (Free Acid)  0.5
Pyridoxine.HCl  0.5
Sucrose  30,000
Thiamine.HCl  0.4

Application Notes
Plant Tissue Culture Tested

Vuylsteke D (1989) Shoot-tip culture for the propagation, conservation, and exchange of Musa germplasm. Practical manuals for handling crop germplasm in vitro. 2. Rome: International Board for Plant Genetic Resources.