Musa (Banana) Multiplication MediumProduct ID: M462
Introduction
Description: Contains the macro- and micronutrients as described by Murashige and Skoog (1962).
IITA formulation as described by Vuylsteke (1989) (International Institute for Tropical Agriculture).
| Solubility |
Water (Partially) |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
36.36 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
M462 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
1650 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
332.2 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.25 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.85 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.74 |
| M250 |
Manganese Sulfate |
16.9 |
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
1900 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
170 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
| A106 |
L-Ascorbic Acid |
20 |
| B800 |
6-Benzylaminopurine (6-BA) |
4.5 |
| G434 |
Gellan Gum (CultureGelT) |
2000 |
| G503 |
Glycine |
2 |
| I885 |
Indole-3-acetic Acid |
0.175 |
| N765 |
Nicotinic Acid |
0.5 |
| P866 |
Pyridoxine HCl |
0.5 |
| S391 |
D-Sucrose |
30,000 |
| T390 |
Thiamine HCl |
0.4 |
M462
Musa (Banana) Multiplication Medium
IITA Formulation*
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Off-White
|
| Application: |
Plant Tissue Culture |
| Solubility: |
Partially Soluble in Cold Water. Fully Soluble in Hot/Boiling Water. |
| Typical Working Concentration: |
36.36 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble
precipitates may form. |
| Other Notes: |
Formulation as described by Vuylsteke (1989).
pH = 3.75 - 4.75
*International Institute for Tropical Agriculture |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
1650 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
332.2 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2EDTA.2H2O |
37.26 |
| Ferrous Sulfate |
27.85 |
| Magnesium Sulfate, Anhydrous |
180.74 |
| Manganese Sulfate.H2O |
16.9 |
| Molybdic Acid (Sodium Salt).2H2O |
0.25 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
1900 |
| Potassium Phosphate Monobasic |
170 |
| Zinc Sulfate.7H2O |
8.6 |
| L-Ascorbic Acid |
20 |
| 6-Benzylaminopurine (BA) |
4.5 |
| Gellan Gum - Biotech Grade |
2000 |
| Glycine |
2 |
| Indole-3-acetic Acid |
0.175 |
| Nicotinic Acid (Free Acid) |
0.5 |
| Pyridoxine.HCl |
0.5 |
| Sucrose |
30,000 |
| Thiamine.HCl |
0.4 |
Application Notes
Plant Tissue Culture Tested
References
Vuylsteke D (1989) Shoot-tip culture for the propagation, conservation, and exchange of Musa germplasm. Practical manuals for handling crop germplasm in vitro. 2. Rome:
International Board for Plant Genetic Resources.
|
|
