Introduction
Description: Finer and Nagasawa Modification.
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: Contains 1.6x Potassium Nitrate and 0.5x Ammonium Nitrate; Ferrous Sulfate and Na2-EDTA is replaced with FeNa-EDTA.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
4.61 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
M504 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
825 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
332.2 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E676 |
Ferric Sodium EDTA |
36.7 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.54 |
| M250 |
Manganese Sulfate |
16.9 |
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
3030 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
170 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
M504
Murashige & Skoog (MS)
Modified Basal Salt Mixture
Finer & Nagasawa Modification
| Properties |
|---|
| Form: |
Fine to Fluffy Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
4.61 g/L |
| Storage Temp: |
2-6° |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the following exceptions: Contains 1.6x Potassium Nitrate and 0.5x Ammonium Nitrate; Ferrous Sulfate and Disodium EDTA is replaced with Ferric Sodium EDTA. |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
825 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
332.2 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Ferric Sodium EDTA |
36.7 |
| Magnesium Sulfate, Anhydrous |
180.54 |
| Manganese Sulfate.H2O |
16.9 |
| Molybdic Acid (Sodium Salt). 2H2O |
0.25 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
3030 |
| Potassium Phosphate, Monobasic |
170 |
| Zinc Sulfate.7H2O |
8.6 |
Application Notes
Plant Tissue Culture Tested
Plant species: Soybean
References
Finer JJ, and A Nagasawa. 1988. Development of an embryogenic suspension culture of soybean (Glycine max Merrill.). Plant Cell Tissue Org Cult 15: 125-136.
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
