Murashige & Skoog Modified Medium (Arabidopsis Culture Medium) with 2,4-D and KinetinProduct ID: M550
Introduction
Description: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins. Also contains (mg/L): 2.0 2,4-D, 0.05 Kinetin, and sucrose.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
24.44 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
M550 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
1650 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
332.2 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.26 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
| M250 |
Manganese Sulfate |
16.9 |
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
1900 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
170 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
| I703 |
myo-Inositol |
100 |
| K750 |
Kinetin |
0.05 |
| N765 |
Nicotinic Acid |
1 |
| P866 |
Pyridoxine HCl |
1 |
| S391 |
D-Sucrose |
20,000 |
| T390 |
Thiamine HCl |
10 |
M550 Murashige & Skoog (MS)Modified Medium Arabidopsis Culture Medium
| Properties |
|---|
| Form: |
Fine to Fluffy Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
24.44g/L |
| Storage Temp: |
2-6° |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and modified vitamins. Also contains (mg/L): 2.0 2,4-D, 0.05 Kinetin, and sucrose |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
1650 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
332.2 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2EDTA.2H2O |
37.26 |
| Ferrous Sulfate.7H2O |
27.8 |
| Magnesium Sulfate, Anhydrous |
180.7 |
| Manganese Sulfate.H2O |
16.9 |
| Molybdic Acid (Sodium Salt). 2H2O |
0.25 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
1900 |
| Potassium Phosphate, Monobasic |
170 |
| Zinc Sulfate.7H2O |
8.6 |
| 2,4-Dichlorophenoxyacetic Acid |
2.0 |
| Kinetin |
0.05 |
| myo-Inositol |
100 |
| Nicotinic Acid (Free Acid) |
1.0 |
| Pyridoxine•HCl |
1.0 |
| Sucrose |
20,000 |
| Thiamine .HCl |
0.4 |
Application Notes
Plant Tissue Culture Tested
Plant species: Arabidopsis thaliana
This medium was developed for the culture of Arabidopsis callus and cell suspension cultures
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497
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