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Murashige & Skoog Basal Salt Mixture, van der Salm Modification
Product ID: M5541


Description: Contains the macro- & micronutrients as described by Murashige & Skoog (1962), and as Modified by Van Der Salm t.m.p. et al. (1994).

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 4.36
Other Notes Plant Tissue Culture Tested
UPC / SKU M5541
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A300 Ammonium Nitrate 1650
B210 Boric Acid 6.2
C266 Calcium Chloride, Anhydrous 332.2
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E676 Ferric Sodium EDTA 96
M150 Magnesium Sulfate, Anhydrous 180.7
M250 Manganese Sulfate 16.9
P840 Potassium Iodide 0.83
P100 Potassium Nitrate 1900
P846 Potassium Phosphate, Monobasic, Anhydrous 170
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 8.6

M5541 Murashige & Skoog (MS) Basal Salt Mixture Van der Salm Modification

Form: Fine Powder
Appearance: Light Burgandy Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.36 g/L
Storage Temp: 2-6°  
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) with the iron source as described by Van der Salm et al. (1994). Unadjusted pH = Approx. 3.5-4.5
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate.H2O 16.9
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Iodide 0.83
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate.7H2O 8.6

Application Notes
Plant Tissue Culture Tested
Plant species: Originally published for use on the rose rootstock cv 'Moneyway' due to chlorosis in shoots cultured on the standard MS formula.

Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.

Van der Salm, TMP, CJG Van der Toorn, CH Hänisch ten Cate, LAM Dubois, DP De Vries, and HJM Dons. 1994. Importance of the iron chelate formula for micropropagation of Rosa Hybrida L. 'Moneyway'. Plant Cell Tiss. and Organ Cult. 37:73-77.