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Mitra Replate/Maintenance Medium
Product ID: M579


Description: For use in orchid culture. Contains the macro- and micro-nutrients, vitamins, and supplements described by Mitra et al. (1976). Also contains Sucrose and Agar.

Solubility Partially Soluble in Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 29.82
Other Notes Plant Tissue Culture Tested
UPC / SKU M579
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A305 Ammonium Sulfate 100
B210 Boric Acid 0.6
C180 Calcium Nitrate 100
C193 Cobalt Nitrate, Hexahydrate 0.05
C375 Cupric Sulfate, Pentahydrate 0.05
E410 EDTA, Disodium Salt 22.3
F263 Ferrous Sulfate, Heptahydrate 16.7
M150 Magnesium Sulfate, Anhydrous 250
M455 Manganese Chloride, Tetrahydrate 0.4
P840 Potassium Iodide 0.03
P100 Potassium Nitrate 180
M651 Sodium Molybdate (VI), Dihydrate 0.05
S515 Sodium Phosphate, Monobasic 150
Z101 Zinc Sulfate, Heptahydrate 0.05
C325 Activated Charcoal 2000
A111 Agar 7000
B140 D-Biotin 0.05
F430 Folic Acid 0.3
N765 Nicotinic Acid 1.25
P866 Pyridoxine HCl 0.3
R779 Riboflavin, Vitamin B2 0.05
S391 D-Sucrose 20,000
T390 Thiamine HCl 0.3

M547 Murashige & Skoog Modified Vitamin Powder (1000x)

Synonym: MS Modified Vitamin Powder

Form: Powder
Appearance: Gray to Black Powder
Application: Orchid Cultur
Solubility: Partially Soluble in Water
Typical Working Concentration: 29.82 g/L
Storage Temp: 2-6° C
Other Notes: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Formula (mg/L)
 Ammonium Sulfate 100
Sodium Phosphate, Monobasic 150
Boric Acid 0.6
Zinc Sulfate, Heptahydrate 0.05
Calcium Nitrate 100
Agar 7,000
Cobalt Nitrate, Hexahydrate 0.05
d-Biotin 0.05
Cupric Sulfate, Pentahydrate 0.05
Charcoal, Activated 2,000
EDTA, Disodium, Dihydrate 22.3
Folic Acid 0.3
Ferrous Sulfate, Hepthydrate 16.7
Nicotinic Acid 1.25
Magnesium Sulfate, Anhydrous 250
Pyridoxine.HCl 0.3
Manganese Chloride, Tetrahydrate 0.4
Riboflavin 0.05
Molybdic Acid, Sodium Salt, Dihydrate 0.05
Sucrose 20,000
Potassium Iodide 0.03
Thiamine.HCl 0.3
Potassium Nitrate 180

Application Notes
Orchid Culture Contains the macro- and micro- nutrients, vitamins, and supplements described by Mitra et al. (1976). Also contains Sucrose and Agar.
Has been used for culture of Vanda sp., Dendrobium sp., and Cymbidium sp.

Mitra et al., 1976. Indian Journal of Experimental Biology. 14:350-351.