Murashige & Skoog Basal Medium with Vitamins, Sucrose, & Gelzan Product ID: M5825
Introduction
Description: Contains the Macro- and Micronutrients and Vitamins as described by Murashige & Skoog (1962). Modified to contain 30 g/L D-Sucrose and 2 g/L Gelzan.
| Solubility |
Partially Soluble in Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
36.43 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
M5825 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
1650 |
| B210 |
Boric Acid |
6.2 |
| C266 |
Calcium Chloride, Anhydrous |
332.2 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.26 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
180.7 |
| M250 |
Manganese Sulfate |
16.9 |
| P840 |
Potassium Iodide |
0.83 |
| P100 |
Potassium Nitrate |
1900 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
170 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
8.6 |
| G3251 |
Gelzan® |
2000 |
| G503 |
Glycine |
2.0 |
| I703 |
myo-Inositol |
100 |
| N765 |
Nicotinic Acid |
0.5 |
| P866 |
Pyridoxine HCl |
0.5 |
| S391 |
D-Sucrose |
30,000 |
| T390 |
Thiamine HCl |
0.1 |
M5825 Murashige & Skoog (MS) Basal Medium w/Sucrose & Gelzan
Synonym: MS Medium w/ Vitamin, Sucrose & Gelzan
| Properties |
|---|
| Form: |
Fine Powder |
| Appearance: |
Tan Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Partially Soluble Water |
| Typical Working Concentration: |
34.43 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Murashige and Skoog (1962). Approximate unadjusted pH = 3.5-5.0 |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
1650 |
| Boric Acid |
6.2 |
| Calcium Chloride, Anhydrous |
332.2 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2EDTA.2H2O |
37.26 |
| Ferrous Sulfate.7H2O |
27.8 |
| Magnesium Sulfate , Anhydrous |
180.7 |
| Manganese Sulfate.H2O |
16.9 |
| Molybdic Acid (Sodium Salt). 2H2O |
0.25 |
| Gelzan |
2000.0 |
| Potassium Iodide |
0.83 |
| Potassium Nitrate |
1900 |
| Potassium Phosphate, Monobasic |
170 |
| Zinc Sulfate.7H2O |
8.6 |
| Glycine(Free Base) |
2 |
| myo-Inositol |
100 |
| Nicotinic Acid (Free Acid) |
0.5 |
| Pyridoxine .HCl |
0.5 |
| Sucrose |
30,000 |
| Thiamine•HCl |
0.1 |
Application Notes
Plant Tissue Culture Tested
Plant species: This medium is used for a numerous species.
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
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