Introduction
Description: Contains the macro- and micronutrients, and organic constituents as described by Lichter (1982). Without sucrose.
This medium was derived from that described by Nitsch & Nitsch and was developed to support the initiation and growth of haploid plants from anther and pollen cultures of Brassica napus. This medium contains the equivalent of 500 mg/L Calcium Nitrate tetrahydrate as described in the original formulation.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
1.77 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
N479 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| B210 |
Boric Acid |
10 |
| C180 |
Calcium Nitrate |
345 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E676 |
Ferric Sodium EDTA |
36.7 |
| M150 |
Magnesium Sulfate, Anhydrous |
61 |
| M250 |
Manganese Sulfate |
18.95 |
| P100 |
Potassium Nitrate |
125 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
125 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
10 |
| B140 |
D-Biotin |
0.05 |
| F430 |
Folic Acid |
0.5 |
| G229 |
L-Glutamine |
800 |
| G394 |
Glutathione (Reduced), Sodium Salt |
30 |
| G503 |
Glycine |
2.0 |
| I703 |
myo-Inositol |
100 |
| N765 |
Nicotinic Acid |
5 |
| P866 |
Pyridoxine HCl |
0.5 |
| S807 |
L-Serine |
100 |
| T390 |
Thiamine HCl |
0.5 |
N479 NLN Basal Medium
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
1.77g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and organic constituents as described by Lichter (1982). |
| Formula (mg/L) |
|---|
| Boric Acid |
10 |
| Calcium Nitrate (dried) |
347 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Ferric Sodium EDTA |
36.7 |
| Magnesium Sulfate, Anhydrous |
61 |
| Manganese Sulfate.H2O |
18.95 |
| Molybdic Acid (Sodium Salt).2H2O |
0.25 |
| Potassium Nitrate |
125 |
| Potassium Phosphate, Monobasic |
125 |
| Zinc Sulfate.7H2O |
10 |
| D-Biotin |
0.05 |
| Folic Acid |
0.5 |
| L-Glutamine |
800 |
| Glutathione |
30 |
| Glycine (Free Base) |
2 |
| myo-Inositol |
100 |
| Nicotinic Acid (Free Acid) |
5 |
| Pyridoxine.HCl |
0.5 |
| L-Serine |
100 |
| Thiamine.HCl |
0.5 |
Application Notes
Plant Tissue Culture Tested Plant species: Brassica napus This medium was derived from that described by Nitsch & Nitsch and was developed to support the initiation and growth of haploid plants from anther and pollen cultures of Brassica napus. This medium contains the equivalent of 500 mg/L calcium nitrate tetrahydrate as described in the original formulation.
References
Lichter, R. 1981. Z. Pflanzenphysiol. 103: 119. Lichter, R. 1982. Z. Pflanzenphysiol. 105: 427
