Introduction
Description: Modified Chu/Gamborg basal medium. Contains the macronutrients described by Chu (1975) & the micronutrients described by Gamborg, et al. (1968).
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
4.1 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
N492 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A305 |
Ammonium Sulfate |
463 |
| B210 |
Boric Acid |
3.0 |
| C266 |
Calcium Chloride, Anhydrous |
125.33 |
| C350 |
Cobalt Chloride Hexahydrate |
0.025 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.26 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
90.37 |
| M250 |
Manganese Sulfate |
10 |
| P840 |
Potassium Iodide |
0.75 |
| P100 |
Potassium Nitrate |
2830 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
400 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
2.0 |
| I703 |
myo-Inositol |
100 |
| N765 |
Nicotinic Acid |
1.0 |
| P866 |
Pyridoxine HCl |
1.0 |
| T390 |
Thiamine HCl |
10 |
N492 NB Basal Medium Modified Chu/ Gamborg Basal Medium
Synonym: WPM Basal Salts
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
4.10g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5 |
| Formula (mg/L) |
|---|
| Ammonium Sulfate |
463 |
| Boric Acid |
3 |
| Calcium Chloride, Anhydrous |
125.33 |
| Cobalt Chloride.6H2O |
0.025 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2 EDTA.2H2O |
37.26 |
| Ferrous Sulfate.7H2O |
27.8 |
| Magnesium Sulfate, Anhydrous |
90.37 |
| Manganese Sulfate.H2O |
10 |
| Molybdic Acid (Sodium Salt).2H2O |
0.25 |
| Potassium Iodide |
0.75 |
| Potassium Nitrate |
2830 |
| Potassium Phosphate, Monobasic |
400 |
| Zinc Sulfate.7H2O |
2 |
| myo-Inositol |
100 |
| Nicotinic Acid (Free Acid) |
1 |
| Pyridoxine.HCl |
1 |
| Thiamine.HCl |
10 |
Application Notes
Plant Tissue Culture Tested
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.
