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NB Basal Medium
Product ID: N492


Description: Modified Chu/Gamborg basal medium. Contains the macronutrients described by Chu (1975) & the micronutrients described by Gamborg, et al. (1968).

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 4.1
Other Notes Plant Tissue Culture Tested
UPC / SKU N492
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
A305 Ammonium Sulfate 463
B210 Boric Acid 3.0
C266 Calcium Chloride, Anhydrous 125.33
C350 Cobalt Chloride Hexahydrate 0.025
C375 Cupric Sulfate, Pentahydrate 0.025
E410 EDTA, Disodium Salt 37.26
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 90.37
M250 Manganese Sulfate 10
P840 Potassium Iodide 0.75
P100 Potassium Nitrate 2830
P846 Potassium Phosphate, Monobasic, Anhydrous 400
M651 Sodium Molybdate (VI), Dihydrate 0.25
Z101 Zinc Sulfate, Heptahydrate 2.0
I703 myo-Inositol 100
N765 Nicotinic Acid 1.0
P866 Pyridoxine HCl 1.0
T390 Thiamine HCl 10

N492 NB Basal Medium Modified Chu/ Gamborg Basal Medium

Synonym: WPM Basal Salts

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 4.10g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Lloyd and McCown (1981). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Sulfate 463
Boric Acid  3
Calcium Chloride, Anhydrous  125.33
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O 37.26
Ferrous Sulfate.7H2O  27.8
Magnesium Sulfate, Anhydrous  90.37
Manganese Sulfate.H2O 10
Molybdic Acid (Sodium Salt).2H2O 0.25
Potassium Iodide  0.75
Potassium Nitrate  2830
Potassium Phosphate, Monobasic  400
Zinc Sulfate.7H2O 2
myo-Inositol  100
Nicotinic Acid (Free Acid) 1
Pyridoxine.HCl 1
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested

Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.

Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.