Introduction
Description: Contains the macro- and micronutrients as described by Nitsch and Nitsch (1969).
This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
2.1 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
N613 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
720 |
| B210 |
Boric Acid |
10 |
| C266 |
Calcium Chloride, Anhydrous |
166 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.26 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
90.372 |
| M250 |
Manganese Sulfate |
18.9 |
| P100 |
Potassium Nitrate |
950 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
68 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
10 |
N613 Nitsch & Nitsch Basal Salt Mixture
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
2.10g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients as described by Nitsch and Nitsch (1969) pH = 3.5 – 4.5 |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
720 |
| Boric Acid |
10 |
| Calcium Chloride, Anhydrous |
166 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2 EDTA.2H2O |
37.26 |
| Ferrous Sulfate.7H2O |
27.8 |
| Magnesium Sulfate, Anhydrous |
90.372 |
| Manganese Sulfate.H2O |
18.9 |
| Molybdic Acid (Sodium Salt).2H2O |
0.25 |
| Potassium Nitrate |
950 |
| Potassium Phosphate, Monobasic |
68 |
| Zinc Sulfate.7H2O |
10 |
Application Notes
Plant Tissue Culture Tested Plant species: Nicotiana (tobacco) This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87
