Introduction
Description: Contains the macro- and micronutrients, and vitamins as described by Nitsch and Nitsch (1969).
This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
2.21 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
N616 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A300 |
Ammonium Nitrate |
720 |
| B210 |
Boric Acid |
10 |
| C266 |
Calcium Chloride, Anhydrous |
166 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.025 |
| E410 |
EDTA, Disodium Salt |
37.26 |
| F263 |
Ferrous Sulfate, Heptahydrate |
27.8 |
| M150 |
Magnesium Sulfate, Anhydrous |
90.372 |
| M250 |
Manganese Sulfate |
18.9 |
| P100 |
Potassium Nitrate |
950 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
68 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.25 |
| Z101 |
Zinc Sulfate, Heptahydrate |
10 |
| B140 |
D-Biotin |
0.05 |
| F430 |
Folic Acid |
0.5 |
| G503 |
Glycine |
2 |
| I703 |
myo-Inositol |
100 |
| N765 |
Nicotinic Acid |
5 |
| P866 |
Pyridoxine HCl |
0.5 |
| T390 |
Thiamine HCl |
0.5 |
N616 Nitsch & Nitsch Basal Medium w/Vitamins
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
2.21g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Nitsch and Nitsch (1969) |
| Formula (mg/L) |
|---|
| Ammonium Nitrate |
720 |
| Boric Acid |
10 |
| Calcium Chloride, Anhydrous |
166 |
| Cupric Sulfate.5H2O |
0.025 |
| Na2 EDTA.2H2O |
37.26 |
| Ferrous Sulfate.7H2O |
27.8 |
| Magnesium Sulfate, Anhydrous |
90.372 |
| Manganese Sulfate.H2O |
18.9 |
| Molybdic Acid (Sodium Salt) .2H2O |
0.25 |
| Potassium Nitrate |
950 |
| Potassium Phosphate, Monobasic |
68 |
| Zinc Sulfate.7H2O |
10 |
| D-Biotin |
0.05 |
| Folic Acid |
0.5 |
| Glycine (Free Base) |
2 |
| myo-Inositol |
100 |
| Nicotinic Acid (Free Acid) |
5 |
| Pyridoxine.HCl |
0.5 |
| Thiamine.HCl |
0.5 |
Application Notes
Plant Tissue Culture Tested
Plant species: Nicotiana (tobacco) This medium was originally developed for the initiation and culture of haploid plants from tobacco microspores. Nitsch & Nitsch has become a popular medium for the haploid culture of many plant species.
References
Nitsch, JP and C Nitsch. 1969. Haploid plants from pollen grains. Science 163: 85-87.
