Introduction
Description: Contains macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan et al. (1987). Contains 10 g/L sucrose. Without vitamins.
This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 µM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse.
| Solubility |
Water (Partially) |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
12.52 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
O622 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| C180 |
Calcium Nitrate |
96.6 |
| M150 |
Magnesium Sulfate, Anhydrous |
23.9 |
| P704 |
Potassium Chloride |
100 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
200 |
| A111 |
Agar |
7000 |
| G386 |
D-Glucose, Anhydrous |
2000 |
| 90988 |
Oats, Rolled |
3000 |
| Y892 |
Yeast Extract |
100 |
O622 H2 OAT MEDIUM
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Cream |
| Application: |
Plant Micropropagation |
| Solubility: |
Partially Soluble in Water |
| Typical Working Concentration: |
12.52 g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of stock solution is not recommended |
| Other Notes: |
Plant tissue culture tested |
| Formula (mg/L) |
|---|
| Calcium Nitrate, Anhydrous |
96.6 |
| Magnesium Sulfate |
23.9 |
| Potassium Chloride |
100 |
| Potassium Phosphate, Monobasic |
200 |
| Yeast Extract |
100 |
| Glucose, Anhydrous |
2000 |
| Rolled Oats |
3000 |
| Agar |
7000 |
Application Notes
Same formulation as prod. # O612 (H1 Oat Medium), but the sucrose is replaced with glucose in this formulation.
Contains the macro- and micronutrients and supplements required for the symbiotic culture of orchids.
