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flg22 Peptide
Product ID: P6622


flg22 Peptide is a peptide with its sequence derived from the flagellin N-terminus of Pseudomonas sp. and is known to elicit specific innate immune response in plants as well as animals. Amino acid sequence: QRLSTGSRINSAKDDAAGLQIA

Solubility Water/DMF
Physical Form Solid
Storage Temp. -20 °C
UPC / SKU P6622
Formula Weight 2272.5 Avg.
Formula C93H162N32O34
Storage Temp. -20 °C
Tariff Code 2930.90.9050
Risk Info (R) 36/37/38
Safety Info (S) 26-36
No information available

P6622 flg22 peptide

Synonyms: flagellin peptide (30-51 aa, P. aeruginosa)
Formula: C93H162N32O34
Theoretical MW: 2272.5 g/mol
Form: Lyophilized powde
Appearance: White to off-white
Solubility: Soluble in sterile water (1 mg/mL) or DMF
Application: Plant Defense and Immunity
Storage Temp: -20°C or below
Typical Working Concentration: 100 pM to 100 nM (Varies with application)

Application Notes
Peptide sequence derived from the flagellin N-terminus of P. aeruginosa that is known to elicit specific innate immune responses in plants as well as animals. It is considered a PAMP (pathogen associated molecular pattern) by its conserved 22-amino acid sequence. In A. thaliana it leads to activation of MAP (mitogen activated protein) kinases as well as activation of PR (pathogenesis-related) genes.

Dissolve in sterile, deionized water. Store at -20°C or below. Aliquot into multiple tubes to avoid multiple freezethaw events. Note peptides and proteins are all susceptible to binding on the surfaces of plastic and glass tubes and bottles and significant losses can be realized during dilutions near or below 10 g/mL. This is a well-known phenomenon for all peptides and proteins and has been seen specifically in the case of flg22 (Felix et al. 1999). To overcome this we would recommend dilutions below 1.0 mg/mL be performed with an aqueous solution of 0.05M NaCl (S624) and 0.1 mg/mL hydrolyzed casein (C184). Bovine serum albumin (BSA) has often been used in the same capacity as hydrolyzed casein, however we recommend hydrolyzed casein due to its widespread use in plant tissue culture.

G. Felix et al. (1999) “Plants have a sensitive perception system for the most conserved domain of bacterial flagellin.” Plant J. Vol. 18(3) pg 265-276. S.T. Chisholm et al. (2006) “Host-Microbe Interactions: Shaping the Evolution of the Plant Immunne Response” Cell Vol 124(4) pg 803-814.