Introduction
Description: A component of Fehling's solution, used to differentiate between water-soluble carbohydrate and ketone functional groups, and as a test for monosaccharides. Fehling's solution can be used to determine whether a carbonyl-containing compound is an aldehyde or a ketone.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| UPC / SKU |
P6780 |
| CAS NUMBER |
6381-59-5 |
| Formula Weight |
282.1 |
| Formula |
KNaC4H4O6 * 4H2O |
| Synonyms |
Fehling's Reagent B, Rochelle Salt, Seignette Salt, Tartaric Acid Potassium Sodium Salt |
| Storage Temp. |
Room Temperature |
| Tariff Code |
2918.13.3000 |
No information available
P6780 Potassium Sodium Tartrate, Tetrahydrate
| Synonyms: |
Rochelle Salt, Seighnette Salt, L(+)-Tartaric acid potassium Sodium salt |
| CAS: |
6381-59-5 |
| Formula: |
282.22 |
| Mol. Weight: |
282.22 |
| Properties |
| Form: |
Powder |
| Appearance: |
White to Off-White |
| Application: |
Seed Testing, Molecular Biology |
| Solubility: |
Soluble in Water |
| Storage Temp: |
Room Temperature |
| Typical Working Concentration: |
34.6g/100 mL |
| Other Notes: |
Fehling’s Reagent (B) |
Application Notes
A component of Fehling's solution, used to differentiate between water-soluble carbohydrate and ketone functional groups, and as a test for monosaccharides. Fehling's solution can be used to determine whether a carbonylcontaining compound is an aldehyde or a ketone.
Fehling's solution, is composed of 2 separately prepared solutions, added together in equal parts.
Fehling Soln A consists of 7 g of hydrated copper (II) sulfate (Prod# C375) dissolved in 100 mL of DI water.
Fehling Soln B is made by dissolving 34.6 g of potassium sodium tartrate and 24 g of sodium hydroxide (Prod # P672) in 100 mL of DI water. This solution is referred to as a general test for monosaccharides (Nigam, 2007) and should always be made fresh.
References
Nigam A. (2007) Lab Manual of Biochemistry. Tata McGraw-Hill Education, ISBN: 0070617678. Pg. 21-22.
Unrau AM (1964) Carbohydrates of Centrosema Seed. The Constitution of an Arabino Galactan. Canadian Journal
of Chemistry, 42. Pp. 916-925.