Introduction
Description: A basal medium used for the selection of common plant pathogens, specifically: Burkholderia glumae, Acidovorax avenae, Pectobacterium carotovorum, Ralstonia solanacearum, and Xanthomonas campestris.
PhytoSelect Basal Medium is formulated based on the SMART media system developed by Kawanishi et al. (2011). SMART is an acronym for: Selective Medium-Design Algorithm Restricted by Two Constraints. The two constraints that are varied to select for different microorganisms are the carbon source and the antibiotics chosen for selection.
This medium is ADP-Free (Animal Derived Products) and includes a gelling agent. It requires the addition of antibiotic(s) and carbon source to be a complete selection medium.For more information on suggested carbon sources and antibiotics, please see the Product Info sheet.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
Room Temperature |
| Grams of powder to prepare 1 Liter |
24.93 |
| UPC / SKU |
P6800 |
| Storage Temp. |
Room Temperature |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A109 |
Ammonium Chloride |
1000 |
| F263 |
Ferrous Sulfate, Heptahydrate |
9 |
| M150 |
Magnesium Sulfate, Anhydrous |
250 |
| P846 |
Potassium Phosphate, Monobasic, Anhydrous |
3000 |
| S515 |
Sodium Phosphate, Monobasic |
5665 |
| A111 |
Agar |
15,000 |
| C1830 |
Crystal Violet |
3 |
P6800 PhytoSelect Basal Medium
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
Off-White, may have slight purple tint |
| Solubility: |
Soluble in Boiling Water |
| Application: |
Seed Testing, Plant Pathology, Microbiology |
| Storage Temp: |
Room Temperature |
| Typical Working Concentration: |
24.93 g/L |
| Other Notes: |
Includes 15 g/L Agar. Contains Crystal Violet. ADP-Free product
Requires addition of antibiotics and a specific carbon source to be a complete selection medium. |
| Formula (g/L) |
|---|
| Ammonium Chloride |
1000 |
| Crystal Violet |
3 |
| Ferrous Sulfate, Heptahydrate |
9 |
| Magnesium Sulfate |
250 |
| Potassium Phosphate |
3000 |
| Sodium Phosphate |
5665 |
| Agar |
15000 |
Application Notes
PhytoSelect Basal Medium is formulated based on the SMART media system developed by Kawanishi et al. (2011). SMART is an acronym for: Selective Medium-Design Algorithm Restricted by Two Constraints. The two constraints that are varied to select for different microorganisms are the carbon source and the antibiotics chosen for selection.
This basal medium contains all the main components found in each of the SMART selection media (does not contain the carbon source or antibiotics). The SMART medium formulation is ADP-Free and uses the same standard components for the basal medium, but varies the carbohydrate source along with the antibiotics to be able to select for different plant pathogens. Kawanishi et al. (2011) report that their developed media have advantages over ADP (Animal Derived Products) containing media, stating that the lack of ADP products allows their formulation to be more selective for particular microorganisms. This reduces the amount of false colonies, and increases the selectivity of the medium.
Kawanishi et al. (2011) have published 5 selection media so far for the following plant pathogens:
Burkholderia glumae, Acidovorax avenae, Pectobacterium carotovorum, Ralstonia solanacearum, and Xanthomonas campestris.
The following page contains a table listing all 5 media formulations, as well as Phytotech's product numbers for the associated components. The antibiotic solutions that we manufacture are sterile and should be added aseptically after autoclaving, when the medium is cool enough to handle.
