ATZ Labs - a subsidiary of Life Technologies (India) Pvt. Ltd.
Life Technologies India
PhytoTechnology Laboratories

Parker Thompson Fern Basal Salt Mixture
Product ID: p713

Introduction

Description: Contains the components as described by Hickok, et al (1995).
This medium was developed for the germination of fern

Solubility Water
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 0.77
Other Notes Plant Tissue Culture Tested
UPC / SKU p713
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A112 Ammonium Molybdate, Tetrahydrate 0.037
A300 Ammonium Nitrate 125
B210 Boric Acid 1.86
C266 Calcium Chloride, Anhydrous 19.628
C375 Cupric Sulfate, Pentahydrate 0.37
E410 EDTA, Disodium Salt 37.3
F263 Ferrous Sulfate, Heptahydrate 27.8
M150 Magnesium Sulfate, Anhydrous 58.565
M250 Manganese Sulfate 0.25
P846 Potassium Phosphate, Monobasic, Anhydrous 500
Z101 Zinc Sulfate, Heptahydrate 0.52

P713 Parker Thompson Fern Basal Salt Mixture

Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 0.77g/L
Storage Temp: 2-6°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Hickok et al. (1995) Unadjusted pH = 3.5 – 4.5 (See certificate of analysis for lot-specific value.)
Formula (mg/L)
Ammonium Molybdate  0.037
Ammonium Nitrate  125
Boric Acid  1.86
Calcium Chloride, Anhydrous 19.628
Cupric Sulfate.5H2O  0.37
Na2 EDTA.2H2O 37.3
Ferrous Sulfate.7H2O 27.8
Magnesium Sulfate, Anhydrous  58.565
Manganese Sulfate.H2O  0.25
Potassium Phosphate, Monobasic 500
Zinc Sulfate.7H2O  0.52

Application Notes
Plant Tissue Culture Tested Plant species: Ceratopteris and other fern species This medium was developed for the germination of fern spores and may need to be supplemented with a carbohydrate source and gelling agent.

References
Hickok, LG, TR Warne, and RS Fribourg. 1995. The biology of the fern Ceratopteris and its use as a model system. Int. J. Plant Sci. 156: 332-345