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Life Technologies India
PhytoTechnology Laboratories

Orchid Seed Sowing Medium II
Product ID: P727

Introduction

Description: This medium is a modification of P723 Orchid Seed Sowing Medium, except charcoal has been eliminated, and is best suited for species that do not produce phenolics. This medium may be used for some terrestrial orchid species by preparing the medium at ½ strength and supplementing it with 50 ml of coconut water per liter.

Solubility Water (Partillay)
Physical Form Solid
Storage Temp. 2-6 °C
Grams of powder to prepare 1 Liter 31.74
Other Notes Plant Tissue Culture Tested
UPC / SKU P727
Storage Temp. 2-6 °C
Tariff Code 3822.00.5090
Risk Info (R) 8-36/37/38
Safety Info (S) 17-26-36
No information available
PROD. # COMPONENT NAME mg/L
A300 Ammonium Nitrate 412.5
B210 Boric Acid 1.65
C266 Calcium Chloride, Anhydrous 83
C350 Cobalt Chloride Hexahydrate 0.0063
C375 Cupric Sulfate, Pentahydrate 0.0063
E410 EDTA, Disodium Salt 18.65
F263 Ferrous Sulfate, Heptahydrate 13.93
M150 Magnesium Sulfate, Anhydrous 75.18
M250 Manganese Sulfate 4.23
P840 Potassium Iodide 0.2075
P100 Potassium Nitrate 475
P846 Potassium Phosphate, Monobasic, Anhydrous 42.5
M651 Sodium Molybdate (VI), Dihydrate 0.0625
Z101 Zinc Sulfate, Heptahydrate 2.65
A111 Agar 8000
I703 myo-Inositol 100
M825 MES, Free Acid 500
N765 Nicotinic Acid 1
P775 Peptone from Meat 2000
P866 Pyridoxine HCl 1
S391 D-Sucrose 20,000
T390 Thiamine HCl 10

P727 Orchid Seed Sowing Medium Contains Agar, Without Charcoal

Properties
Form: Powder
Appearance: Off-white to Cream Powde
Application: Orchid Culture
Solubility: Hot Water
Typical Working Concentration: 31.74 g/L
Storage Temp: 2-6° C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: The pH of this medium should be adjusted prior to sterilization.
412.5
Formula (mg/L)
Ammonium Nitrate 
Boric Acid  1.65
Calcium Chloride, Anhydrous  83
Cobalt Chloride.6H2O  0.0063
Cupric Sulfate.5H2O  0.0063
Na2EDTA.2H2O  18.65
Ferrous Sulfate.7H2O  13.93
Magnesium Sulfate,Anhydrous  75.18
Manganese Sulfate.H2O  4.23
Molybdic Acid, Sodium Salt.2H2O  0.0625
Potassium Iodide  0.2075
Potassium Nitrate  475
Potassium Phosphate, Monobasic 42.5
Zinc Sulfate.7H2O 2.65
Agar 8000
MES (Free Acid) 500
myo-Inositol 100
Nicotinic Acid (Free Acid) 1
Peptone from Meat 2000
Pyridoxine.HCl 1
Sucrose  20,000
Thiamine.HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Phalaenopsis, Epiphytic orchids
This medium is a modification of P723 Orchid Seed Sowing Medium, ex cept charcoal has been eliminated, and is best suited for s pecies that do not produce phenolics.
This medium may be used for some terrestrial orchid species by preparing the medium at ½ strength and supplementing it with 50 ml of coconut water per liter. Approximately 4 g/L of agar should also be added. (Revised 12/2012)