Introduction
Description: Staba Modified Vitamin Solution (100x) contains a modification of the Staba (1969) vitamins as described by Skirvin and Chu (1979). This modification differs from the original Staba formulation in that myo-Inositol was reduced from 2000 mg/L to 100 mg/L. The original use of these vitamins appears to be for the culture of Digitalis. These vitamins have been used for the culture of Rubus (Blackberry), Prunus (Peach), Rosa (Rose), Cornus (Dogwood), Rhipsalidopsis (Easter Cactus), and Schlumbergera (Christmas Cactus).
myo-Inositol may precipitate out of the solution when cold; warming with occasional agitation will redissolve it. Use at a concentration of 10 mL/L of medium to achieve the proper final concentration.
| Miscibility |
Water |
| Physical Form |
Liquid |
| Concentration |
100x |
| Sterility |
Sterile Filtered |
| Storage Temp. |
-20-0 °C |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
S743 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A103 |
?-Aminobenzoic Acid |
50 |
| B140 |
D-Biotin |
100 |
| C186 |
Calcium Pantothenate |
100 |
| C232 |
Choline Chloride |
100 |
| C242 |
Cyanocobalamin, Vitamin B12 |
0.15 |
| F430 |
Folic Acid |
50 |
| I703 |
myo-Inositol |
10,000 |
| N609 |
Niacinamide |
200 |
| P866 |
Pyridoxine HCl |
200 |
| R779 |
Riboflavin, Vitamin B2 |
50 |
| T390 |
Thiamine HCl |
100 |
S743
Staba Modified Vitamin Solution
(100x)
| Properties |
|---|
| Form: |
Liquid |
| Appearance: |
Clear Solution |
| Application: |
Plant Tissue Culture |
| Solubility: |
Miscible with Water |
| Typical Working Concentration: |
1 mL/ L of medium |
| Storage Temp: |
-20-0° C |
| Storage Temp of Stock Solution: |
N/A |
| Other Notes: |
Contains a modification of the Staba (1969) vitamins as described by Skirvin and Chu (1979). This modification differs from the original Staba formulation in that myo-inositol was reduced from 2000 mg/L to 100 mg/L.
Myo-Inositol may precipitate out of the solution when cold; warming with occasional agitation will redissolved it. |
| Formula (mg/L) |
|---|
| ?-Aminobenzoic Acid |
50 |
| D-Biotin |
100 |
| Calcium Pantothenate |
100 |
| Choline Chloride |
100 |
| Cyanocobalamin |
0.15 |
| Folic Acid |
50 |
| myo-Inositol |
10,000 |
| Niacinamide |
200 |
| Pyridoxine HCl |
200 |
| Riboflavin |
50 |
| Thiamine HCl |
100 |
Application Notes
Plant Tissue Culture Tested
The original use of these vitamins appears to be for the culture of Digitalis. These vitamins have
been used for the culture of Rubus (Blackberry), Prunus (Peach), Rosa (Rose), Cornus
(Dogwood), Rhipsalidopsis (Easter Cactus), and Schlumbergera (Christmas Cactus).
References
Staba, JE. 1969. Plant tissue culture as a technique for the phytochemist. Recent Adv. in Phytochem., 2:80.
Skirvin, RM and MC Chu. 1979. In vitro culture of 'Forever Yours' rose. HortSci., 14: 608-610. (Revised 5/2007)
