Schenk & Hildebrandt Modified Basal Medium w/ Vitamins & Sucrose Product ID: S813
Introduction
Description: Contains the macro- and micronutrients and vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose.
| Solubility |
Water |
| Physical Form |
Solid |
| Storage Temp. |
2-6 °C |
| Grams of powder to prepare 1 Liter |
14.21 |
| Other Notes |
Plant Tissue Culture Tested |
| UPC / SKU |
S813 |
| Storage Temp. |
2-6 °C |
| Tariff Code |
3822.00.5090 |
| Risk Info (R) |
8-36/37/38 |
| Safety Info (S) |
17-26-36 |
No information available
| PROD. # |
COMPONENT NAME |
mg/L |
| A113 |
Ammonium Phosphate |
300 |
| B210 |
Boric Acid |
5 |
| C266 |
Calcium Chloride, Anhydrous |
151 |
| C350 |
Cobalt Chloride Hexahydrate |
0.1 |
| C375 |
Cupric Sulfate, Pentahydrate |
0.2 |
| E410 |
EDTA, Disodium Salt |
20 |
| F263 |
Ferrous Sulfate, Heptahydrate |
15 |
| M150 |
Magnesium Sulfate, Anhydrous |
195.4 |
| M250 |
Manganese Sulfate |
10 |
| P840 |
Potassium Iodide |
1.0 |
| P100 |
Potassium Nitrate |
2500 |
| M651 |
Sodium Molybdate (VI), Dihydrate |
0.1 |
| Z101 |
Zinc Sulfate, Heptahydrate |
1.0 |
| I703 |
myo-Inositol |
1000 |
| N765 |
Nicotinic Acid |
5.0 |
| P866 |
Pyridoxine HCl |
0.5 |
| S391 |
D-Sucrose |
10,000 |
| T390 |
Thiamine HCl |
5.0 |
S813 Schenk & Hildebrandt Modified Basal Medium Contains Vitamins and 10 g/L Sucrose
| Properties |
|---|
| Form: |
Powder |
| Appearance: |
White to Yellow Powder |
| Application: |
Plant Tissue Culture |
| Solubility: |
Water |
| Typical Working Concentration: |
14.21g/L |
| Storage Temp: |
2-6°C |
| Storage Temp of Stock Solution: |
Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: |
Contains the macro- and micronutrients and vitamins as described by Schenk and Hildebrandt (1972). Also contains 10 g/L Sucrose. pH = 3.75 – 4.75 |
| Formula (mg/L) |
|---|
| Ammonium Phosphate, Monobasic |
300 |
| Boric Acid |
5 |
| Calcium Chloride, Anhydrous |
151 |
| Cobalt Chloride.6H2O |
0.1 |
| Cupric Sulfate.5H2O |
0.2 |
| Na2 EDTA.2H2O |
20 |
| Ferrous Sulfate.7H2O |
15 |
| Magnesium Sulfate, Anhydrous |
195.4 |
| Manganese Sulfate.H2O |
10 |
| Molybdic Acid (Sodium Salt).2H2O |
0.1 |
| Potassium Iodide |
1 |
| Potassium Nitrate |
2500 |
| Zinc Sulfate.7H2O |
1 |
| myo-Inosito |
1000 |
| Nicotinic Acid (Free Acid) |
5 |
| Pyridoxine•HCI |
0.5 |
| Sucrose |
10,000 |
| Thiamine•HCl |
5 |
Application Notes
Plant Tissue Culture Tested
References
Schenk, RU & AC Hildebrandt. 1972. Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Can. J. Bot. 50: 199-204.
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